核酸检测在寨卡病毒病诊断上的应用

发布时间：2018-10-16 15:05

【摘要】：目的研究核酸检测技术测定不同样本对我国输入性寨卡病毒(Zika virus)病患者的诊断价值,探讨核酸检测寨卡病毒临床标本的适用性。方法收集医院2016年2月-10月输入性寨卡病毒病患者的血液、唾液和尿液,用于寨卡病毒核酸检测。记录和分析患者临床症状、体征和实验室检验结果。结果血液、唾液和尿液寨卡核酸首次检测阳性率分别为61.5%、76.9%和76.9%;血液、唾液和尿液的核酸总检出阳性率分别为61.5%、84.6%和100%;血液、唾液和尿液核酸阳性持续时间平均分别为2.62、5.69和11.62天,血液没有1例阳性持续时间超过1周,唾液阳性持续时间没有1例超过10天,而尿液有11例核酸阳性超过1周(其中6例超过10天)。随着病程的发展,各样本的检出率也在下降,发病后第3天和第7天采样时,血液、唾液、尿液核酸阳性率分别为25%、100%、100%和7.7%、46.2%、84.6%。少数尿液标本检测阳性有一定滞后现象。结论检测尿液中寨卡病毒核酸具有检出率高、持续时间长的优点,应作为临床诊断寨卡病毒病的主要方法,但在发病早期仍然有必要采集血液、唾液和尿液进行多样本同时检测寨卡病毒核酸。
[Abstract]:Objective to study the diagnostic value of nucleic acid detection technique in patients with imported Zika virus (Zika virus) disease in China, and to explore the applicability of nucleic acid detection for Zika virus clinical specimens. Methods Blood, saliva and urine of imported Zika virus patients from February to October 2016 were collected for detection of Zika virus nucleic acid. Record and analyze clinical symptoms, signs and laboratory results. Results the positive rates of Zika nucleic acid in blood, saliva and urine were 76.9% and 76.9% respectively, the total positive rates of nucleic acid in blood, saliva and urine were 84.6% and 100, respectively, and the mean positive duration of nucleic acid in blood, saliva and urine were 2.625.69 and 11.62 days, respectively. None of the patients had a positive duration of more than 1 week, none of them had a positive duration of more than 10 days in saliva, while 11 cases of urine were positive for nucleic acid for more than 1 week (6 of them were more than 10 days). The positive rates of nucleic acid in blood, saliva and urine were 25100% and 7.7% respectively on the 3rd and 7th day after the onset of the disease. A few urine samples tested positive have some lag phenomenon. Conclusion Detection of Zika virus nucleic acid in urine has the advantages of high detection rate and long duration. It should be used as the main method for clinical diagnosis of Zika virus disease, but it is still necessary to collect blood in the early stage of the disease. Multiple samples of saliva and urine were used to detect Zika virus nucleic acids at the same time.
【作者单位】：广东省江门市疾病预防控制中心;江门市恩平市人民医院感染科;江门市恩平市疾病预防控制中心;
【基金】：江门市科技计划基金资助项目(2016-5-8)
【分类号】：R511

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