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PCR检测与细菌培养方法在细菌性痢疾监测中的应用效果对比研究

发布时间:2018-10-16 16:24
【摘要】:目的:研讨细菌性痢疾患者接受聚合酶链反应(PCR)检测及细菌培养法检测的临床价值。方法:从我院2015年1月-2016年10月选取580例腹泻患者参与研究试验,依次对上述患者行细菌培养、PCR技术检查,评估该两种检测方案对志贺菌的检测情况。结果:PCR检测结果呈阳性者294例,检出率50.7%(294/580),相比细菌培养法的4.8%(28/580)显著提高,数据满足P0.05,统计学成立。且不同年龄(除≥70岁)检出情况相比,PCR的检出率均显著比细菌培养法高,数据满足P0.05,统计学成立。结论:与细菌培养法相比,PCR检测技术对细菌性痢疾具有更准确的评估作用,值得加强推广。
[Abstract]:Objective: to study the clinical value of polymerase chain reaction (PCR) and bacterial culture in patients with bacillary dysentery. Methods: from January 2015 to October 2016, 580 patients with diarrhea were selected to take part in the study. The bacteria culture and PCR technique were used to evaluate the detection of Shigella. Results: the positive rate of PCR was 50.7% (294 / 580), which was significantly higher than that of bacterial culture (4.8%, 28 / 580). The detection rate of PCR was significantly higher than that of bacterial culture method except for 鈮,

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