大麻素受体1与FAK在血吸虫肝纤维化小鼠肝组织中的表达及意义

发布时间：2018-10-20 14:48

【摘要】：目的：探讨大麻素受体1（cannabionoid receptor1,CB1）与粘着斑激酶（focaladhesion kinase,FAK）在血吸虫肝纤维化小鼠肝组织中的表达及变化情况，进一步探讨血吸虫肝纤维化的发生及发展机制。方法：清洁级雄性昆明小鼠共40只，随机分为正常组和模型组，正常组10只，模型组30只，模型组小鼠用腹壁贴附尾蚴法建立血吸虫肝纤维化小鼠模型，，均普通喂养，8周后将小鼠全部处死并取肝组织备用，根据纤维化程度将模型组分为三组，HE染色观察小鼠肝组织病理变化，Masson染色观察肝纤维化程度，免疫组织化学方法检测各组肝组织CB1表达，RT-PCR检测各组肝组织CB1mRNA及FAK mRNA的表达情况。结果： 1、8周末，HE染色及Masson染色结果发现，正常肝组织结构正常，模型组其正常肝组织结构被破坏，血吸虫虫卵沉积，且其周围有大量纤维结缔组织及炎性细胞，小鼠血吸虫肝纤维化形成，造模成功。 2、与正常组比较，模型组均有虫卵结节及不同程度的纤维化改变（P＜0.01）；不同纤维化程度组其虫卵结节及门静脉周围均可见CB1、CB1mRNA及FAK mRNA明显表达，其差别具有统计学意义（P＜0.01）。 3、随着肝脏纤维化程度的增加，CB1、CB1mRNA及FAK mRNA的表达亦相应增加(P＜0.05或＜0.01) 结论: 1、大麻素受体1有促进血吸虫肝纤维化形成的作用。 2、其形成机制及途径可能与FAK活化，进一步激活PI3K有关。
[Abstract]:Aim: to investigate the expression and changes of cannabinoid receptor 1 (cannabionoid receptor1,CB1) and adhesion kinase (focaladhesion kinase,FAK) in liver tissue of mice with schistosomiasis hepatic fibrosis, and to explore the mechanism of hepatic fibrosis of Schistosoma japonicum. Methods: forty male Kunming mice of clean grade were randomly divided into normal group (n = 10) and model group (n = 30). After 8 weeks, all the mice were killed and the liver tissues were taken for preparation. According to the degree of fibrosis, the model group was divided into three groups. The pathological changes of liver tissue were observed by HE staining, and the degree of hepatic fibrosis was observed by Masson staining. Immunohistochemical method was used to detect the expression of CB1 in liver tissue and RT-PCR was used to detect the expression of CB1mRNA and FAK mRNA in liver tissue. Results: 1 at the end of 8 weeks, the results of HE staining and Masson staining showed that the normal liver tissue structure was normal, the normal liver tissue structure was destroyed, the eggs of Schistosoma japonicum were deposited, and a large number of fibrous connective tissues and inflammatory cells were found around them. The hepatic fibrosis of Schistosoma japonicum in mice was successfully established. 2. Compared with normal group, the model group had egg nodule and fibrosis in varying degrees (P < 0.01). The expression of CB1,CB1mRNA and FAK mRNA were observed in the egg nodules and around the portal vein in the groups with different degrees of fibrosis. The difference was statistically significant (P < 0. 01). 3. With the increase of liver fibrosis, the expression of CB1,CB1mRNA and FAK mRNA also increased (P < 0. 05 or < 0. 01). Conclusion: 1. Cannabinoid receptor 1 can promote blood. The mechanism and pathway of hepatic fibrosis induced by paragonimiasis may be related to the activation of FAK. Further activation of PI3K related to.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R575.2;R532.21

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