丙型肝炎病毒样颗粒作为亚甲蓝光化学法灭活HCV效果评价物的可行性研究
发布时间：2018-11-01 19:58
【摘要】：目的探讨丙型肝炎病毒样颗粒(HCVLPs)在亚甲蓝光化学灭活处理过程中的变化趋势及其作为亚甲蓝光化学法灭活HCV效果评价物的可行性。方法以HCV阳性血浆作为平行对照组(HCV RNA载量约6.53 logcopies/m L)(n=6),将HCVLPs用代血浆调整成合适浓度(HCV RNA定量约为6.74 logcopies/m L)的悬液作为实验组(n=5),将2组分装至PVC血袋中,MB+L 1μmol/L进行病毒灭活处理,分别于光照处理0、5、10、20、30 min时取样,用荧光定量PCR技术检测病毒核酸载量;同时以细胞病变法测定HCV模型病毒sindbis病毒的残余滴度,验证MB+L灭活HCV的效果。结果模型病毒sindbis的病毒滴度降至检测限以下(log TCID50/m L≤0.5);MB+L处理过程中,随着光照处理时间的增加,HCV及HCVLPs的核酸载量明显下降,分别从6.53与6.74下降至4.51和2.89log copies/m L(P0.05),且2组在不同取样点的HCV RNA载量之间具有相关性(R20.98,Significance F0.01)。结论 HCVLPs能够反映MB+L灭活处理中HCV RNA动态变化,或有望成为安全而有效的HCV灭活评价物来监控HCV灭活效果。
[Abstract]:Objective to investigate the change trend of hepatitis C virus like particle (HCVLPs) in methylene blue photochemical inactivation and its feasibility as an evaluation of the efficacy of methylene blue photochemical inactivation of HCV. Methods the HCV positive plasma was used as the parallel control group, the (HCV RNA load was about 6.53 logcopies/m L) (/ nm6), and the HCVLPs substitute plasma was adjusted to the appropriate concentration of (HCV RNA (about 6.74 logcopies/m L) as the experimental group (N5). The two groups were divided into PVC blood bag for inactivation of, MB L 1 渭 mol/L. The viral nucleic acid load was detected by fluorescence quantitative PCR technique at 10 ~ 20 ~ 30 min after light treatment. At the same time, the residual titer of HCV model virus sindbis virus was determined by cytopathic method to verify the effect of MB L inactivation of HCV. Results the virus titer of the model virus sindbis decreased below the detection limit (log TCID50/m L 鈮，
本文编号：2304944
[Abstract]:Objective to investigate the change trend of hepatitis C virus like particle (HCVLPs) in methylene blue photochemical inactivation and its feasibility as an evaluation of the efficacy of methylene blue photochemical inactivation of HCV. Methods the HCV positive plasma was used as the parallel control group, the (HCV RNA load was about 6.53 logcopies/m L) (/ nm6), and the HCVLPs substitute plasma was adjusted to the appropriate concentration of (HCV RNA (about 6.74 logcopies/m L) as the experimental group (N5). The two groups were divided into PVC blood bag for inactivation of, MB L 1 渭 mol/L. The viral nucleic acid load was detected by fluorescence quantitative PCR technique at 10 ~ 20 ~ 30 min after light treatment. At the same time, the residual titer of HCV model virus sindbis virus was determined by cytopathic method to verify the effect of MB L inactivation of HCV. Results the virus titer of the model virus sindbis decreased below the detection limit (log TCID50/m L 鈮，
本文编号：2304944
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