荧光RT-PCR检测与ELISA检测在戊肝临床诊断效果的比较分析

发布时间：2018-11-03 11:40

【摘要】：目的探讨和比较荧光RT-PCR检测与ELISA检测在戊肝临床诊断的效果。方法从我院健康体检中心2013年1月至2016年1月的50500血清标本中随机选择100份作为研究标本。通过采用酶联免疫法对戊肝抗体的检测,然后再通过荧光RT-PCR检测法进行确诊,观察和比较两组检测方法的检查结果。结果阳性一致率为55%,阴性一致率为100%,总一致率为75%,Kappa值为0.59(0.4Kappa≤0.75),表示两者一致性一般。ELISA阳性检出率为30%,PCR阳性检出率为50%,PCR阳性检出率明显高于ELISA,数据比较差异具有统计学意义,P=0.0000.005。结论荧光RTPCR检测和ELISA检测在戊肝的诊断中都具有一定的检查准确度,同时荧光RT-PCR检测的阳性检出率明显高于ELISA检测。由于ELISA检测容易受到各方面因素的影响而发生漏诊的情况,因此建议对于ELISA检测出现可疑的标本,使用RT-PCR进行确诊,使更具可靠性。
[Abstract]:Objective to investigate and compare the clinical diagnosis of hepatitis E by fluorescence RT-PCR and ELISA. Methods from January 2013 to January 2016, 50500 serum samples were randomly selected. The antibody against hepatitis E was detected by enzyme-linked immunosorbent assay (Elisa), and then confirmed by fluorescence RT-PCR assay. The results of the two methods were observed and compared. Results the positive consistency rate was 55, the negative consistency rate was 100 and the total consistency rate was 75. 59 (0.4Kappa 鈮，

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