输血传播恶性疟疾实验室检测和溯源分析——附报告1例

发布时间：2018-11-03 12:29

【摘要】：目的通过对有输血史的恶性疟疾感染者进行检测和溯源分析,确定其传染源和传播方式。方法对患者和为其输血的8名献血者血样进行疟疾快速检测、血涂片镜检和Real-time PCR检测,并对阳性血样进行分子溯源和基因分型。结果患者与1名非洲返乡献血者在疟疾快速诊断试剂检测、血涂片镜检和Real-time PCR 3项检测中,均显示为恶性疟阳性。两者血样SSU rRNA基因序列两两比对后同源性为100%,Pf EMP-1基因分型显示受血者和献血者血样均为恶性疟原虫K1型和MD20型混合感染。确定该患者的恶性疟确因输入非洲返乡献血者的血液而感染。讨论由于我国未将疟疾筛查列入献血前检测,因此境外返乡人员在疟疾潜伏期或复发期内参加献血易引起输血传播疟疾。采供血机构需仔细征询既往病史和外出史,并加强宣教,将经血传播的疾病控制在源头。
[Abstract]:Objective to determine the source of infection and transmission of falciparum malaria infected by blood transfusion. Methods the blood samples of the patients and 8 blood donors were tested for malaria, blood smear microscopic examination and Real-time PCR, and the positive blood samples were analyzed by molecular tracing and genotyping. Results both the patient and a homegrown blood donor from Africa showed falciparum malaria positive in rapid diagnostic test, blood smear microscopic examination and Real-time PCR test. The homology of the two SSU rRNA gene sequences was 100% PF EMP-1 genotyping showed that the blood samples of both recipients and donors were mixed infection of Plasmodium falciparum K1 and MD20 type. Confirmed that the patient's falciparum malaria was infected by transfusion of blood from returning blood donors in Africa. It is discussed that malaria screening is not included in the blood donation test in China, so it is easy to cause blood transfusion transmission malaria during the latent period or relapse of malaria donation. Blood collecting and supplying organizations should carefully consult the past medical history and travel history, and strengthen propaganda and education to control the blood-borne diseases at their source.
【作者单位】：丽水市中心血站;丽水市疾病预防控制中心;
【分类号】：R457.1;R531.3

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