脑膜炎奈瑟菌4种聚合酶链反应检测方法的评价

发布时间：2018-11-05 14:19

【摘要】：目的对脑膜炎奈瑟菌(Neisseria meningitidis,Nm)检测中常用的聚合酶链反应(Polymerase Chain Reaction,PCR)方法进行适用性评价。方法选取130株不同血清群Nm及109株其他种属细菌,通过PCR检测荚膜转移基因A(Capsule Transport A,ctr A)、铜-锌超氧化物歧化酶(Cu-Zn Superoxide Dismutase C,sod C)和lysR家族转录调节基因A(LysR-family Transcriptional Regulator A,crg A),其中,荧光实时(Real Time)-PCR(RT-PCR)检测ctr A基因(ctr A-RT)和sod C基因(sod C-RT),普通PCR检测crg A(crg A)和sod C(sod C);并采用281份脑脊液(Cerebrospinal Fluid,CSF)标本和162份健康人群咽拭子标本对两种RT-PCR检测方法进行了比较。结果在菌株检测中,ctr A-RT法能检测出所有可分群Nm菌株和大部分不可分群菌株,未检出其他菌株;sod C-RT、crg A和sod C法除检测出所有Nm菌株,还能检测出部分其他菌株。在CSF标本检测中,ctr A-RT和sod C-RT法结果完全一致。在咽拭子标本检测中,ctr A-RT和sod C-RT法符合率低。结论 4种PCR检测方法中,单独采用任何1种,均不能将Nm与其他细菌完全区分开。建议根据不同的标本选择适宜的检测方法。
[Abstract]:Objective to evaluate the applicability of polymerase chain reaction (Polymerase Chain Reaction,PCR) in the detection of Neisseria meningitidis (Neisseria meningitidis,Nm). Methods 130 strains of Nm and 109 strains of other bacteria belonging to different serogroups were selected to detect Cu-Zn Superoxide Dismutase C, by PCR for A (Capsule Transport ACTR A), copper and zinc superoxide dismutase (Cu-Zn Superoxide Dismutase C,). Ctr A (ctr A-RT) and sod C (sod C-RT) were detected by real-time (Real Time) PCR (RT-PCR) in sod C) and lysR family. Detection of crg A (crg A) and sod C (sod C); by ordinary PCR The detection methods of RT-PCR were compared between 281 cerebrospinal fluid (Cerebrospinal Fluid,CSF) specimens and 162 pharyngeal swabs of healthy people. Results in the detection of strains, ctr A-RT method could detect all the Nm strains and most of the non-separable strains, but no other strains were detected. Sod C-RTG A and sod C methods not only detected all Nm strains, but also some other strains. The results of ctr A-RT and sod C-RT were identical in CSF specimens. In the detection of throat swabs, the coincidence rate of ctr A-RT and sod C-RT was low. Conclusion none of the four PCR detection methods can completely distinguish Nm from other bacteria. It is suggested to select suitable detection methods according to different specimens.
【作者单位】：中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室;
【基金】：国家科学技术艾滋病和病毒性肝炎等重大传染病防治十二五重大专项基于全基因组序列的细菌分型技术课题(编号:2013ZX10004221) 国家科学技术重点基础研究发展计划(973)重要病原体变异规律与致病机制研究课题(编号2011CB504900)
【分类号】：R515.2;R440

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