雷丸及吡喹酮对猬裂头蚴感染小鼠的疗效观察

发布时间：2018-11-07 11:23

【摘要】：目的了解雷丸及吡喹酮对猬裂头蚴疗效的影响。方法 (1)从黑斑蛙体内检获裂头蚴。将112只昆明小鼠分成14组(A-I),每只小鼠经口感染裂头蚴5条,每组8只小鼠,每组共感染裂头蚴40条。(2)感染后1周,A-C组小鼠分别用吡喹酮灌胃治疗1个疗程(1次/d×3 d),吡喹酮1个疗程的总剂量分别为1 500 mg/kg、3 000mg/kg和6 000 mg/kg;D-F组分别用雷丸灌胃治疗1个疗程(1次/d×3 d),雷丸1个疗程的总剂量分别为1 200mg/kg、2 400 mg/kg和4 800 mg/kg。(3)H-J组和K-M组小鼠分用吡喹酮和雷丸治疗一个疗程后,间隔7 d,再治疗1个疗程,H-J组的总剂量同A-C组,K-M组的总剂量同D-F组;G组和N组小鼠仅灌服生理盐水,分别作为A-F组和H-M组小鼠的对照组。所有处理组及对照组在疗程结束后1周剖杀,分别计数每组小鼠的裂头蚴检获数及减虫率。结果 (1)1个疗程治疗:A组及B组小鼠与G对照组相比,裂头蚴的减虫率差异无统计学意义(P0.05);C组小鼠裂头蚴的平均检获数为2.13条,裂头蚴的减虫率为41.32%,与G对照组相比,差异有统计学意义(P0.05);D组、E组及F组小鼠裂头蚴的减虫率分别为48.21%、51.79%和44.90%,与G对照组相比,差异均有统计学意义(P0.05)。(2)2个疗程的治疗:H、I和J组与N对照组相比,裂头蚴的减虫率差异无统计学意义(P0.05);K、L和M组小鼠裂头蚴的减虫率分别为48.22%、55.62%和59.17%,与N对照组相比,差异均有统计学意义(P0.05),雷丸各治疗组之间裂头蚴的减虫率差异无统计学意义(P0.05)。结论雷丸和吡喹酮在体内对猬裂头蚴感染小鼠有一定的治疗作用,雷丸的疗效优于吡喹酮。
[Abstract]:Objective to investigate the effect of Lei Pill and praziquantel on the efficacy of hedgehog mitomycaria. Methods (1) the cleft cercariae were seized from the body of the black spot frog. 112 Kunming mice were divided into 14 groups (A-I). Each mouse was infected with 5 mitomycaria by mouth, 8 mice in each group. (2) one week after infection, each group was infected with 40 mitomycaria. A-C mice were treated with praziquantel for a course of treatment (1 time / d 脳 3 d), praziquantel) with total doses of 1 500 mg/kg,3 000mg/kg and 6 000 mg/kg;, respectively. In D-F group, the total dose of Lei Pill was 1 200 mg / kg, and the total dose of one course was 1 200 mg / kg per day 脳 3 d),. After 2 400 mg/kg and 4 800 mg/kg. (3) H-J and K-M groups were treated with praziquantel and Lei Pill, the mice were treated with praziquantel and Lei Pill at intervals of 7 days for another course of treatment. The total dose of H-J group was the same as that of A-C group. The total dose of K-M group was the same as that of D-F group. Group G and group N were treated with saline only as control group of A-F group and H-M group. All the treatment group and control group were dissected 1 week after the course of treatment and counted the number of caterpillars seized and the rate of worm reduction in each group. Results (1) A course of treatment: compared with G control group, there was no significant difference in worm reduction rate between group A and group B (P0.05). The average seizure number was 2.13 in group C, and the worm reduction rate was 41.32 in group C, which was significantly different from that in group G (P0.05). The worm reduction rates of group D, group E and group F were 48.21%, 51.79% and 44.90%, respectively. Compared with group G, the difference was statistically significant (P0.05). (2) for two courses of treatment: H. There was no significant difference in worm reduction rate between group I and group J compared with control group (P0.05). The deworming rates of the mitomycaria in the KG L and M groups were 48.2222% and 59.17.2%, respectively, which were significantly different from those in the N control group (P0.05). There was no significant difference in worm reduction rate among the treatment groups (P0.05). Conclusion Lei Pill and praziquantel have some therapeutic effects on mice infected with Hedgehog mitomycaria in vivo, and Lei Pill is better than praziquantel.
【作者单位】：贵阳医学院人体寄生虫学教研室;
【基金】：贵州省科技厅基金项目支助[黔科合J字(2012)2042号]
【分类号】：R532.3

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