查尔酮类化合物L2H17对甲型流感病毒FM1株感染小鼠的保护作用
发布时间：2018-11-13 18:28
【摘要】：目的研究查尔酮类化合物(代号:L2H17,简称L2)对流感病毒感染小鼠的保护作用及引起的病毒性肺炎的治疗作用。方法将小鼠随机分为6组:正常组、模型组、阳性药奥司他韦(达菲)组(20 mg/kg)、L2 20、40、80mg/kg剂量组,适应性喂养72 h,滴鼻(IN)造模,于造模前24 h给药,连续给药6 d观察至14 d,统计各组小鼠的中位生存时间、死亡保护率和生命延长率,观察L2对流感病毒感染小鼠的保护作用;按上法于造模后第3、5天无菌条件取小鼠全肺,计算肺指数及肺指数抑制率,左肺用4%甲醛固定,用于病理组织切片;右肺浸泡于RNAstore,检测肺组织病毒载量;小鼠摘眼球取血,采用双抗体夹心ELISA法检测小鼠血清中炎症细胞因子IL-6、TNF-α含量,观察L2对流感病毒感染引起的病毒性肺炎的治疗作用。结果与模型组相比,L2 80 mg/kg剂量组死亡率下降50%,中位生存时间、死亡保护率、生命延长率均有所提高;造模后第3、5天,L2 80 mg/kg剂量组肺指数降低,病理形态也有所改善;小鼠肺组织病毒载量降低(P0.05);炎症因子IL-6含量较模型组有不同程度的降低。结论L2对流感病毒感染小鼠具有一定的保护作用,能减轻流感病毒所致肺炎的病变程度。
[Abstract]:Objective to study the protective effect of chalcone compounds (code name: L2H17, L2) on mice infected with influenza virus and its therapeutic effect on viral pneumonia. Methods mice were randomly divided into 6 groups: normal group, model group, positive drug oseltamivir (Tamiflu) group (20 mg/kg), L220 + 400.80mg / kg group. The mice were fed adaptively for 72 h and were given nasal (IN) 24 hours before the model was made. The median survival time, death protection rate and life prolongation rate of each group were counted to observe the protective effect of L2 on mice infected with influenza virus. The whole lungs of the mice were collected according to the aseptic condition on the 3rd day after modeling. The lung index and the inhibition rate of lung index were calculated. The left lung was fixed with 4% formaldehyde for pathological tissue section, and the right lung was immersed in RNAstore, to detect the viral load in the lung tissue. The serum inflammatory cytokines (IL-6,TNF- 伪) in mice were detected by double antibody sandwich ELISA method, and the therapeutic effect of L2 on viral pneumonia caused by influenza virus infection was observed. Results compared with the model group, the mortality of the L280 mg/kg group was decreased by 50%, the median survival time, the death protection rate and the life prolongation rate were increased. The lung index of L280 mg/kg group was decreased and the pathological morphology was improved. The viral load of mouse lung tissue was decreased (P0.05); the content of inflammatory factor IL-6 was decreased in different degree compared with the model group. Conclusion L 2 has protective effect on mice infected with influenza virus and can reduce the severity of pneumonia caused by influenza virus.
【作者单位】： 山东中医药大学药学院;军事医学科学院放射与辐射医学研究所;温州医科大学药学院化学生物学研究中心;河南中医药大学;
【基金】：国家自然科学基金重点资助项目(81230089);国家自然科学基金面上项目(81473184,31270197)
【分类号】：R511.7
，
本文编号：2330062
[Abstract]:Objective to study the protective effect of chalcone compounds (code name: L2H17, L2) on mice infected with influenza virus and its therapeutic effect on viral pneumonia. Methods mice were randomly divided into 6 groups: normal group, model group, positive drug oseltamivir (Tamiflu) group (20 mg/kg), L220 + 400.80mg / kg group. The mice were fed adaptively for 72 h and were given nasal (IN) 24 hours before the model was made. The median survival time, death protection rate and life prolongation rate of each group were counted to observe the protective effect of L2 on mice infected with influenza virus. The whole lungs of the mice were collected according to the aseptic condition on the 3rd day after modeling. The lung index and the inhibition rate of lung index were calculated. The left lung was fixed with 4% formaldehyde for pathological tissue section, and the right lung was immersed in RNAstore, to detect the viral load in the lung tissue. The serum inflammatory cytokines (IL-6,TNF- 伪) in mice were detected by double antibody sandwich ELISA method, and the therapeutic effect of L2 on viral pneumonia caused by influenza virus infection was observed. Results compared with the model group, the mortality of the L280 mg/kg group was decreased by 50%, the median survival time, the death protection rate and the life prolongation rate were increased. The lung index of L280 mg/kg group was decreased and the pathological morphology was improved. The viral load of mouse lung tissue was decreased (P0.05); the content of inflammatory factor IL-6 was decreased in different degree compared with the model group. Conclusion L 2 has protective effect on mice infected with influenza virus and can reduce the severity of pneumonia caused by influenza virus.
【作者单位】： 山东中医药大学药学院;军事医学科学院放射与辐射医学研究所;温州医科大学药学院化学生物学研究中心;河南中医药大学;
【基金】：国家自然科学基金重点资助项目(81230089);国家自然科学基金面上项目(81473184,31270197)
【分类号】：R511.7
，
本文编号：2330062
本文链接：https://www.wllwen.com/yixuelunwen/chuanranbingxuelunwen/2330062.html
最近更新
教材专著
