HBV基因变异对荧光定量PCR系统检测HBV-DNA准确性的影响

发布时间：2018-11-15 23:15

【摘要】：目的探讨乙型肝炎病毒(HBV)基因型和病毒变异对Roche公司COBAS AmpliPrep/COBAS TaqMan 48系统和荧光定量PCR系统检测HBV-DNA的影响。方法收集医院肝病中心2014年3月-2015年10月HBV感染患者100例,明确HBV基因型和变异情况,同时采用Roche公司COBAS AmpliPrep/COBAS TaqMan48系统和荧光定量PCR系统检测HBV-DNA,比较针对不同HBV基因型和病毒变异时两种方法检测结果的差异和相关性。结果两种方法检测B型、C型患者的病毒载量差异均无统计学意义,对未发生病毒变异的患者,两种方法的检测结果差异无统计学意义;对发生病毒变异的患者,两种方法的检测结果差异有统计学意义(t=2.29,P=0.028);两种方法检测B、C型HBV-DNA时的相关系数r分别为0.82、0.86,差异无统计学意义;两种方法检测未发生病毒变异患者HBV-DNA的相关系数r为0.91,显著高于发生病毒变异时的相关系数(r=0.70)(Z=3.13,P0.01)。结论对于不同的HBV基因型,Roche公司COBAS AmpliPrep/COBAS TaqMan系统和国产荧光定量PCR系统在检测HBV-DNA方面有较好的相关性,但HBV基因变异可能会影响后者的准确性。
[Abstract]:Objective to investigate the effect of hepatitis B virus (HBV) genotype and virus variation on the detection of HBV-DNA by Roche COBAS AmpliPrep/COBAS TaqMan 48 system and fluorescence quantitative PCR system. Methods 100 patients with HBV infection from March 2014 to October 2015 were collected from Hepatology Center of Hospital. The genotype and variation of HBV were determined. Meanwhile, HBV-DNA, was detected by Roche COBAS AmpliPrep/COBAS TaqMan48 system and fluorescence quantitative PCR system. The differences and correlations between the two methods for different HBV genotypes and virus mutation were compared. Results there was no significant difference in viral load between the two methods in patients with type B and type C, but there was no significant difference between the two methods in the patients without virus variation. There was a significant difference between the two methods in the detection of HBV-DNA (t = 2.29, P 0.028), and the correlation coefficient (r) of the two methods for detecting HBV-DNA of type C was 0.82 卤0.86, respectively, and there was no significant difference between the two methods. The correlation coefficient (r = 0.91) between the two methods for the detection of HBV-DNA in patients without virus mutation was significantly higher than that at the time of virus mutation (r = 0.70) (P 0.01). Conclusion for different HBV genotypes, Roche COBAS AmpliPrep/COBAS TaqMan system and domestic fluorescent quantitative PCR system have good correlation in HBV-DNA detection, but HBV gene variation may affect the accuracy of the latter.
【作者单位】：宁波市第二医院肝病科;宁波市第二医院检验科;
【基金】：浙江省医药卫生省部培育计划(2014PYA018) 宁波市社发重大项目(2016C51005)
【分类号】：R512.62

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