2008-2013年福建省HIV-1毒株耐药基因变异研究

发布时间：2018-11-17 10:32

【摘要】：目的分析2008-2013年福建HIV-1毒株耐药基因变异情况。方法收集2008-2013年福建省病毒载量大于1 000copy/mL艾滋病患者,采用自建HIV-1基因型耐药检测进行耐药检测和分析。结果共收集226例病毒载量大于1 000copy/mL样本,平均载量值1.62×105copy/mL;124例样本扩增及测序后获得蛋白酶以及部分反转录酶基因序列,亚型分析显示92例(74.2%)为CRF_01AE亚型,14例(11.3%)为B亚型,C和BC亚型各有7例,另4例为其他亚型。77对反转录酶以及蛋白酶抑制剂有不同程度耐药;对核苷类和非核苷类反转录酶抑制剂的耐药分别有63例(50.8%)和74例(59.7%),对蛋白酶抑制剂耐药9例(7.3%);核苷类反转录酶抑制剂主要耐药突变包括了M184V、M41L、D67N、L70R、T219F、K219Q等,造成对3TC、AZT、D4T等不同程度耐药;非核苷类反转录酶抑制主要耐药突变包括K101E、Y181C、K103N、G190A等,造成对NVP和EFV不同程度耐药。结论福建省HIV-1感染者总体耐药率较低,但是随着治疗人数增加以及治疗时间延长,对反转录酶抑制剂出现较高程度耐药,仍需加强艾滋病患者耐药检测。
[Abstract]:Objective to analyze the variation of drug resistance genes of HIV-1 strains from 2008 to 2013 in Fujian Province. Methods patients with HIV / AIDS were collected from 2008 to 2013 in Fujian province. The drug resistance of HIV-1 genotypes was detected and analyzed. Results A total of 226 virus samples were collected with an average load value of 1.62 脳 105 copies per mL. The sequence of protease and partial reverse transcriptase gene was obtained from 124 samples. The subtype analysis showed that 92 cases (74.2%) were CRF_01AE subtype, 14 cases (11.3%) were B subtype, and 7 cases were C and BC subtype, respectively. The other 4 cases were other subtypes. 77 were resistant to reverse transcriptase and protease inhibitors to different degrees. 63 cases (50.8%) and 74 cases (59.7%) were resistant to nucleoside and non-nucleoside reverse transcriptase inhibitors, and 9 cases (7.3%) were resistant to protease inhibitors. The major drug resistance mutations of nucleoside reverse transcriptase inhibitors included M1844 VX M41LD67NNN L70RGN T219FK219Q and so on, resulting in resistance to 3TCU AZTN D4T and so on. Non-nucleoside reverse transcriptase inhibits major drug resistance mutations including K101EY181CU K103NG190A resulting in resistance to NVP and EFV to varying degrees. Conclusion the overall drug resistance rate of HIV-1 infected patients in Fujian Province is low, but with the increase of the number of patients treated and the prolongation of treatment time, there is a high degree of resistance to reverse transcriptase inhibitors, so it is still necessary to strengthen the detection of drug resistance in AIDS patients.
【作者单位】：福建省疾病预防控制中心;
【分类号】：R512.91

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