血浆miR-122在抗-HCV阳性献血者中表达的动态分析
发布时间：2018-11-18 21:02
【摘要】：目的动态分析抗-HCV阳性献血者血浆miR-122的表达特点及与HCV感染诊断指标的关联。方法对133名抗-HCV阳性献血者、24名健康献血者及15名确证抗-HCV阳性追踪献血者血液标本抽提血浆miRNA,以实时荧光定量PCR法检测血浆miR-122,分析血浆miR-122相对表达量与HCV RNA、抗-HCV临界值指数(COI)等指标关联性。结果 ROC曲线分析:miR-122在抗-HCV+/HCV RNA+组和抗-HCV+/HCV RNA-组中诊断丙型肝炎的AUC(ROC曲线下面积)为0.830(95%CI:0.755-0.889),区分抗-HCV+/HCV RNA+组和抗-HCV+/HCV RNA-组的敏感性和特异性分别为74.7%和82.4%。追踪15例抗-HCV+/HCV RNA+献血者血浆miR-122表达明显降低(P0.05)。结论血浆miR-122与HCV感染密切相关,确证HCV RNA感染献血者血浆miR-122随时间迁移表达降低。
[Abstract]:Objective to dynamically analyze the expression of plasma miR-122 in anti-HCV positive blood donors and its association with HCV infection diagnosis. Methods Plasma miRNA, was extracted from 133 blood samples of anti HCV positive blood donors, 24 healthy blood donors and 15 confirmed anti HCV positive blood donors. The real-time fluorescence quantitative PCR method was used to detect plasma miR-122,. The correlation between the relative expression of plasma miR-122 and the critical value index (COI) of HCV RNA, anti-HCV was analyzed. Results ROC curve analysis: the area under AUC (ROC curve of miR-122 in anti HCV / HCV RNA group and anti HCV / HCV RNA- group was 0.830 (95%CI:0.755-0.889). The sensitivity and specificity of differentiating anti-HCV / HCV RNA and anti-HCV / HCV RNA- were 74.7% and 82.4%, respectively. The expression of plasma miR-122 was significantly decreased in 15 anti-HCV / HCV RNA donors (P 0.05). Conclusion there is a close relationship between plasma miR-122 and HCV infection. It is confirmed that the expression of plasma miR-122 decreases with time migration in HCV RNA infected blood donors.
【作者单位】： 深圳市血液中心;
【基金】：深圳市科技创新委员会项目(JCYJ20140403093211510) 深圳市科卫生计生系统科研项目(201401074,201401077)
【分类号】：R512.63
，
本文编号：2341219
[Abstract]:Objective to dynamically analyze the expression of plasma miR-122 in anti-HCV positive blood donors and its association with HCV infection diagnosis. Methods Plasma miRNA, was extracted from 133 blood samples of anti HCV positive blood donors, 24 healthy blood donors and 15 confirmed anti HCV positive blood donors. The real-time fluorescence quantitative PCR method was used to detect plasma miR-122,. The correlation between the relative expression of plasma miR-122 and the critical value index (COI) of HCV RNA, anti-HCV was analyzed. Results ROC curve analysis: the area under AUC (ROC curve of miR-122 in anti HCV / HCV RNA group and anti HCV / HCV RNA- group was 0.830 (95%CI:0.755-0.889). The sensitivity and specificity of differentiating anti-HCV / HCV RNA and anti-HCV / HCV RNA- were 74.7% and 82.4%, respectively. The expression of plasma miR-122 was significantly decreased in 15 anti-HCV / HCV RNA donors (P 0.05). Conclusion there is a close relationship between plasma miR-122 and HCV infection. It is confirmed that the expression of plasma miR-122 decreases with time migration in HCV RNA infected blood donors.
【作者单位】： 深圳市血液中心;
【基金】：深圳市科技创新委员会项目(JCYJ20140403093211510) 深圳市科卫生计生系统科研项目(201401074,201401077)
【分类号】：R512.63
，
本文编号：2341219
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