CD11b与CD27定义的T细胞亚群与HIV疾病进展的相关性研究

发布时间：2018-11-19 10:56

【摘要】：目的探究CD11b与CD27定义的T细胞亚群与HIV疾病进展的相关性,为研究HIV感染者T细胞免疫缺陷提供新的思路。方法外周血荧光抗体染色,用流式细胞仪检测CD11b、CD27及各亚群的表达;破膜胞内染色检测各亚群分泌IFN-γ的功能;细胞因子与PBMC共培养,检测CD11b、CD27的变化。结果在HIV-1感染者,CD27~+CD11b~-、CD27~+CD11b~+和CD27~-CD11b~+的T细胞亚群明显降低(P0.05),而CD27~-CD11b~-T细胞亚群明显增多(P0.05),该亚群与CD4~+T细胞呈显著的负相关(r=-0.545,P0.001);CD27~+CD11b~-、CD27~+CD11b~+和CD11b~+CD27~-亚群分泌IFN-γ功能均强于CD27~-CD11b~-亚群;细胞因子TGF-β随着质量浓度的增加对CD11b表达的抑制也逐渐增强,而IL-12和IL-15能够刺激CD11b的增多,IL-2与IL-7能够刺激CD27增多。结论 HIV感染导致人体T细胞亚群发生紊乱。
[Abstract]:Objective to explore the correlation between T cell subsets defined by CD11b and CD27 and the progression of HIV disease, and to provide a new idea for the study of T cell immune deficiency in HIV infected patients. Methods Peripheral blood fluorescent antibody staining was used to detect the expression of CD11b,CD27 and its subsets by flow cytometry, the function of IFN- 纬 secreted by each subgroup was detected by membrane breaking staining, and the changes of CD11b,CD27 were detected by co-culture of cytokines and PBMC. Results T cell subsets of CD27~ CD11b~-,CD27~ CD11b~ and CD27~-CD11b~ decreased significantly in HIV-1 infected patients (P0.05), while CD27~-CD11b~-T subsets increased significantly (P0.05). There was a significant negative correlation between this subgroup and CD4~ T cells (r-0.545, P0.001). The function of IFN- 纬 secreted by CD27~ CD11b~-,CD27~ CD11b~ and CD11b~ CD27~- subsets was stronger than that of CD27~-CD11b~- subsets. The inhibitory effect of cytokine TGF- 尾 on the expression of CD11b increased with the increase of mass concentration, while IL-12 and IL-15 stimulated the increase of CD11b and IL-2 and IL-7 stimulated the increase of CD27. Conclusion HIV infection leads to disorder of T cell subsets.
【作者单位】：中国医科大学附属第一医院检验科卫生部艾滋病免疫学重点实验室;
【基金】：国家科技重大专项课题(2014ZX10001002001004) 基础临床紧密结合平台项目(医大发字[2013]5号) 辽宁省艾滋病转化医学研究中心建设项目(2015225013)
【分类号】：R512.91

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