血吸虫果糖二磷酸醛缩酶诱导Th2免疫偏移的研究
发布时间：2018-11-27 12:04
【摘要】：目的分析日本血吸虫感染以及虫源性糖蛋白果糖二磷酸醛缩酶(Sj FBPA)刺激后小鼠脾脏细胞中白介素4(IL-4)、白细胞分化抗原CD8a因子表达量的动态变化,并探讨Sj FBPA与辅助型T细胞(Th2)免疫应答的关系。方法血吸虫尾蚴感染C57小鼠10只,设正常对照组。感染8周后处死小鼠,取脾脏制备淋巴细胞悬液;用藻红蛋白(PE)标记CD8a抗体,藻青蛋白(APC)标记IL-4抗体进行细胞染色;流式细胞仪检测细胞因子表达量的变化。10只正常C57小鼠处死后取脾脏制成淋巴细胞悬液,加Sj FBPA蛋白体外刺激,按上述流程刺激方法检测细胞。结果小鼠感染血吸虫8周后脾细胞中IL-4、CD8a的表达量分别为(22.39±5.93)%、(11.34±2.15)%,正常对照组脾细胞中IL-4、CD8a的表达量分别为(1.39±0.52)%、(1.98±0.84)%,差异有统计学意义(P0.01)。Sj FBPA体外刺激小鼠脾细胞的IL-4、CD8a的表达量分别为(14.14±1.16)%、(8.99±0.81)%,正常对照组脾细胞中IL-4、CD8a的表达量分别为(1.86±0.93)%、(1.69±0.56)%,差异有统计学意义(P0.01)。结论 Sj FBPA在血吸虫感染中具有诱导Th2免疫偏移的作用,树突状细胞作为提呈细胞参与该免疫反应。
[Abstract]:Objective to analyze the dynamic changes of expression of interleukin 4 (IL-4) and leukocyte differentiation antigen (CD8a) in spleen cells of mice after Schistosoma japonicum infection and (Sj FBPA) stimulation with insect-derived glycoprotein fructose diphosphate aldolase (Sj FBPA). To investigate the relationship between Sj FBPA and the immune response of helper T cells (Th2). Methods 10 C 57 mice infected with cercariae of Schistosoma japonicum were set up as normal control group. After 8 weeks of infection, the mice were killed and the spleen was taken to prepare lymphocyte suspensions, the CD8a antibody was labeled with phycoerythrin (PE) and the IL-4 antibody was stained with phycocyanin (APC). The expression of cytokines in 10 normal C57 mice was detected by flow cytometry. The spleen of 10 normal C57 mice was taken to make lymphocyte suspension and stimulated by Sj FBPA protein in vitro. Results the expression of IL-4,CD8a in spleen cells was (22.39 卤5.93)% and (11.34 卤2.15)% respectively after 8 weeks of schistosomiasis infection in mice, and the expression of IL-4,CD8a in splenocytes of normal control group was (1.39 卤0.52)%. The expression of IL-4,CD8a was (14.14 卤1.16)% and (8.99 卤0.81)%, respectively. The expression of IL-4,CD8a was (1.86 卤0.93)% and (1.69 卤0.56)% in the normal control group, respectively. The difference was statistically significant (P0.01). Conclusion Sj FBPA can induce Th2 immune deviation in Schistosoma japonicum infection, and dendritic cells are involved in the immune response as presenting cells.
【作者单位】： 安徽医科大学第一附属医院检验科;安徽人畜共患病研究所;
【基金】：国家自然科学基金(编号:81171606)
【分类号】：R532.21
[Abstract]:Objective to analyze the dynamic changes of expression of interleukin 4 (IL-4) and leukocyte differentiation antigen (CD8a) in spleen cells of mice after Schistosoma japonicum infection and (Sj FBPA) stimulation with insect-derived glycoprotein fructose diphosphate aldolase (Sj FBPA). To investigate the relationship between Sj FBPA and the immune response of helper T cells (Th2). Methods 10 C 57 mice infected with cercariae of Schistosoma japonicum were set up as normal control group. After 8 weeks of infection, the mice were killed and the spleen was taken to prepare lymphocyte suspensions, the CD8a antibody was labeled with phycoerythrin (PE) and the IL-4 antibody was stained with phycocyanin (APC). The expression of cytokines in 10 normal C57 mice was detected by flow cytometry. The spleen of 10 normal C57 mice was taken to make lymphocyte suspension and stimulated by Sj FBPA protein in vitro. Results the expression of IL-4,CD8a in spleen cells was (22.39 卤5.93)% and (11.34 卤2.15)% respectively after 8 weeks of schistosomiasis infection in mice, and the expression of IL-4,CD8a in splenocytes of normal control group was (1.39 卤0.52)%. The expression of IL-4,CD8a was (14.14 卤1.16)% and (8.99 卤0.81)%, respectively. The expression of IL-4,CD8a was (1.86 卤0.93)% and (1.69 卤0.56)% in the normal control group, respectively. The difference was statistically significant (P0.01). Conclusion Sj FBPA can induce Th2 immune deviation in Schistosoma japonicum infection, and dendritic cells are involved in the immune response as presenting cells.
【作者单位】： 安徽医科大学第一附属医院检验科;安徽人畜共患病研究所;
【基金】：国家自然科学基金(编号:81171606)
【分类号】：R532.21
【共引文献】
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