东方田鼠抗日本血吸虫病相关机理研究
发布时间：2019-01-08 18:43
【摘要】：目的： 血吸虫病是由血吸虫感染引起的人兽共患病,是一种分布广泛、严重危害人类健康的寄生虫病。本研究以天然抗血吸虫病的东方田鼠为动物模型进行研究。对感染血吸虫后第6、10、15、20、30天东方田鼠肝和肺组织进行病理学观察,并对东方田鼠肝脏转录组基因进行测序,结合文献从中筛选出与东方田鼠天然抗日本血吸虫感染相关免疫分子基因和生长发育相关分子基因,并对这些基因在东方田鼠感染血吸虫前及感染后不同时间点表达水平进行检测,意图进一步阐明东方田鼠对日本血吸虫的天然抗性机理。 方法： 取10周龄东方田鼠30只,随机分为6组,每组5只。第一组为空白对照组,[第2-6组分别感染日本血吸虫尾蚴,感染剂量为2000条／只。分别取空白对照组东方田鼠和感染血吸虫后第6、10、15、20、30天东方田鼠肺、肝、脾组织样品,HE染色后做病理学观察。 取四只10周龄正常东方田鼠,取新鲜肝脏组织,提取总RNA用SMART对东方田鼠肝脏cDNA进行测序,并统计其生物学水平,细胞学水平,分子学水平基因分布。 通过测序结果结合文献,筛选出与东方田鼠天然抗日本血吸虫病相关基因,溶菌酶基因(Lyz)、MHCⅡ类分子基因(RT1-Db1)、CD74基因(Cd74)、补体1q基因(Clqa)、甲状腺素受体a基因(Thra)、胰岛素样生长因子1基因(Igfl),并对这6个基因在东方田鼠感染血吸虫前及感染后第6、10、15、20、30天表达水平变化进行检测。 结果： 东方田鼠感染血吸虫后第6-10天肉眼可见肺组织出现大量出血点,肺体轻度增大,第15天开始出血点消失；第10-20天肝组织表面出现散在白点,肝体轻度增大,颜色呈暗红色,第30天恢复正常。光镜切片观察显示第6-10天肺组织出现严重病理损伤,局部有出血点,毛细血管扩张、管壁增厚,支气管和血管周围及管腔内有大量炎性细胞,其中以嗜酸性粒细胞为主,第20天后开始恢复；第6-20天肝细胞空泡变性,肝窦高度扩张,肝间质、血管周围及虫体周围均有大量嗜酸性粒细胞及少量中性粒细胞、巨噬细胞等炎性细胞浸润,局部出现灶性肝细胞坏死,第30天后恢复。 东方田鼠肝脏cDNA测序结果揭示了东方田鼠cDNA序列,并对这些序列进行生物学水平,细胞学水平,分子学水平功能分类,并从中确定了与东方田鼠天然抗日本血吸虫相关基因Lyz、RT1-Db1、Cd74、Clqa、Thra、Igf1基因序列。 东方田鼠肺内感染血吸虫后,Lyz、RT1-Db1、Cd74基因在肺内第6-10天显著上调(P0.01),第15天起逐渐恢复至正常表达水平；Lyz、RT1-Db1、Cd74基因在肝内第10-15天表达显著上调(P0.01),第20天起逐渐恢复至正常表达水平。Clqa基因在肺内第6-10天表达显著上调(P0.01),第15天起逐渐恢复至正常表达水平,肝内表达无变化。Thra基因在肺内第10-15天表达显著下调(P0.01),第20天起逐渐恢复至正常水平；肝内表达水平无变化；Igf1基因在肺内表达水平无变化；肝内第10-15天表达显著下调(P0.01),第20天起逐渐恢复至正常水平。
[Abstract]:Purpose: Schistosomiasis is a common disease of the human animal caused by the infection of the schistosome, which is a parasite that is widely distributed and is seriously harmful to human health The study is based on the study of the animal model of the animal model with the natural anti-schistosomiasis. The liver and lung tissues of Microtus fortis were observed in the 6th, 10th, 15th, 20th and 30th day after the infection of Schistosoma japonicum, and the liver transcriptome gene of the Microtus fortis was sequenced. The expression of these genes at different time points before and after the infection of Schistosoma japonicum was detected with the intention of further elucidating the natural resistance of the Microtus fortis to the Schistosoma japonicum. Daniel. Methods: 30-week-old Microtus voles were randomly divided into 6 groups. 5 rats in each group. The first group was the blank control group.[Group 2-6] was infected with the tail of Schistosoma japonicum, and the infection dose was 20. (6), 10, 15, 20 and 30 days after infection with the control group, the lung, the liver, the spleen tissue samples and the HE staining of the oriental voles in the blank control group, respectively. Pathological observation. Four 10-week-old Microtus fortis were taken, fresh liver tissue was taken, and the total RNA was extracted with SMART to sequence the cDNA of the liver of the Microtus fortis, and the biological level, the level of the cytology, the molecule, The gene, lysozyme gene (Lyz), MHC class 鈪，
本文编号：2404981
[Abstract]:Purpose: Schistosomiasis is a common disease of the human animal caused by the infection of the schistosome, which is a parasite that is widely distributed and is seriously harmful to human health The study is based on the study of the animal model of the animal model with the natural anti-schistosomiasis. The liver and lung tissues of Microtus fortis were observed in the 6th, 10th, 15th, 20th and 30th day after the infection of Schistosoma japonicum, and the liver transcriptome gene of the Microtus fortis was sequenced. The expression of these genes at different time points before and after the infection of Schistosoma japonicum was detected with the intention of further elucidating the natural resistance of the Microtus fortis to the Schistosoma japonicum. Daniel. Methods: 30-week-old Microtus voles were randomly divided into 6 groups. 5 rats in each group. The first group was the blank control group.[Group 2-6] was infected with the tail of Schistosoma japonicum, and the infection dose was 20. (6), 10, 15, 20 and 30 days after infection with the control group, the lung, the liver, the spleen tissue samples and the HE staining of the oriental voles in the blank control group, respectively. Pathological observation. Four 10-week-old Microtus fortis were taken, fresh liver tissue was taken, and the total RNA was extracted with SMART to sequence the cDNA of the liver of the Microtus fortis, and the biological level, the level of the cytology, the molecule, The gene, lysozyme gene (Lyz), MHC class 鈪，
本文编号：2404981
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