外周感染流感病毒WSN33对小鼠自发行为的影响

发布时间：2019-01-10 19:09

【摘要】：目的:研究外周感染流感病毒H1N1 WSN33后,小鼠在旷场自发行为中的变化规律,探讨外周感染流感病毒对小鼠情绪的影响。方法:通过滴鼻感染H1N1 WSN33或生理盐水,记录BALB/c小鼠两周内的体重变化,以及在旷场行为箱体内5 min的行走总路程、平均运动速度、中央区域运动路程及粪便排泄情况等。结果:外周感染H1N1 WSN33引起小鼠体重急速下降,从第8天开始逐渐恢复。旷场试验中WSN33感染组小鼠行走总路程减少,在感染后的5到10 d总路程减少量差异性比较显著;平均运动速度无统计学差异性;粪便粒数波动较大,无统计学差异。结论:外周感染流感病毒H1N1 WSN33后,BALB/c小鼠自发活动量减少,活动速度基本不变,启示流感病毒感染过程中伴随一定程度的抑郁、焦虑、紧张等情绪,在流感病毒感染的急性期和恢复初期表现比较明显,而紧张情绪表现不太显著。
[Abstract]:Aim: to study the changes of open field spontaneous behavior of mice infected with influenza virus (H1N1 WSN33), and to explore the effect of peripheral influenza virus infection on emotion of mice. Methods: the body weight changes of BALB/c mice during two weeks were recorded by nasal drip of H1N1 WSN33 or normal saline, and the total walk distance, average speed of movement, movement distance of central area and excretion of feces in open field behavior box for 5 min were recorded. Results: peripheral infection with H1N1 WSN33 caused rapid weight loss in mice and gradually recovered from the 8th day. In the open field test, the total distance of WSN33 infected mice decreased, and the total distance decreased significantly from 5 to 10 days after infection. There was no significant difference in average exercise speed; the number of fecal grains fluctuated greatly, and there was no statistical difference. Conclusion: after peripheral infection with influenza virus H1N1 WSN33, the spontaneous activity of BALB/c mice decreases, and the speed of activity is basically unchanged, which indicates that the influenza virus infection is accompanied by a certain degree of depression, anxiety, tension and other emotions. The symptoms of acute and early recovery of influenza virus infection were obvious, but the symptoms of stress were not significant.
【作者单位】：汕头大学医学院微生物学与免疫学教研室广东省感染病与分子免疫病理重点实验室;
【基金】：国家自然科学基金(31170852,81001322,31300761) 广东高校优秀青年教师培养计划(Yq2013077)资助
【分类号】：R511.7

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