广州地区病毒性腹泻的病原分布和病毒基因分型调查

发布时间：2019-03-28 13:06

【摘要】：目的初步了解广州地区儿童和成人病毒性腹泻的病原学构成和分布情况及相关病毒的分子特征。方法收集2012年11月-2013年5月于珠江医院就诊的腹泻患者的粪便标本,采用免疫层析法检测轮状病毒和腺病毒抗原,实时荧光PCR检测诺如病毒GI/GII,上述检测阳性标本进行病毒基因测序分型。结果 290例患者中,3种常见病毒的检出率分别为21.4%、15.5%和1.7%;男性患者病毒阳性率显著高于女性患者(χ2=0.017,P0.05);≤5岁患者轮状病毒阳性率最高,为28.49%(χ2=0.017,P0.05),5岁患者诺如病毒阳性率最高,为21.62%;轮状病毒性腹泻秋冬季节高发;轮状病毒G/P分型以G1P8、G9P8、G2P4和G3P8为主,占92.16%,诺如病毒共检测出8种基因型,以GII.4型为主,占53.33%;病毒合并其他常见腹泻病原体感染占病毒阳性标本的15.89%。结论轮状病毒和诺如病毒分别是广州地区婴幼儿和成人病毒性腹泻的主要病原体,本地区流行的轮状病毒和诺如病毒优势株分别是G1P8和GII.4,诺如病毒基因型多样性更丰富;混合感染常见,需引起重视。
[Abstract]:Objective to investigate the etiology and distribution of viral diarrhea in children and adults and the molecular characteristics of the virus in Guangzhou. Methods fecal specimens of diarrhea patients from Pearl River Hospital from November 2012 to May 2013 were collected. Rotavirus and adenovirus antigens were detected by immunochromatography, and norovirus GI/GII, was detected by real-time fluorescence PCR. The positive samples were sequenced and typed. Results in 290 patients, the positive rate of three common viruses was 21.4%, 15.5% and 1.7%, respectively, and the positive rate of virus in male patients was significantly higher than that in female patients (蠂 ~ 2 / 0.017, P0.05). The positive rate of rotavirus was the highest in 鈮，

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