结核诊断中应用T细胞斑点试验的效果分析

发布时间：2019-04-29 17:06

【摘要】：目的分析结核分枝杆菌T细胞斑点试验(T-SPOT.TB)技术在结核性胸膜炎诊断中的诊断效果,为临床诊断提供参考依据。方法选取2012年5月-2015年5月在医院就诊的结核性胸膜炎患者192例为观察组,选取同期非结核性胸腔积液患者190例为对照组,对结核分枝杆菌早期分泌靶抗原6(ESAT-6)和(或)培养虑液蛋白10(CFP-10)致敏的T细胞,并与PPD皮内试验、腺苷脱氢酶(ADA)、胸水结核分枝杆菌聚合酶链反应(TB-DNA)进行比较,数据采用SPSS 19.0软件进行统计分析。结果观察组患者ESAT-6、CFP-10、E/C阳性率分别为85.4%、75.0%,91.7%,均高于对照组,差异均有统计学意义(P0.05);T-SPOT.TB(E/C)敏感度、约登指数、诊断准确率明显高于T-SPOT.TB(ESAT-6)和T-SPOT.TB(CFP-10);T-SPOT.TB(E/C)诊断敏感度、特异度、约登指数及诊断准确率优于其他方法,经统计检验,差异有统计学意义(P0.05)。结论结核分枝杆菌T细胞斑点试验技术在结核性胸膜炎诊断敏感度、特异度和准确率较高。
[Abstract]:Objective to analyze the diagnostic effect of Mycobacterium tuberculosis T cell dot test (T-SPOT.TB) in the diagnosis of tuberculous pleurisy and to provide reference for clinical diagnosis. Methods 192 patients with tuberculous pleurisy from May 2012 to May 2015 were selected as observation group and 190 patients with non-tuberculous pleural effusion as control group. Sensitized T cells sensitized by Mycobacterium tuberculosis early secretory target antigen 6 (ESAT-6) and / or liquid protein 10 (CFP-10), and intradermal test with PPD, adenosine dehydrogenase (ADA), Mycobacterium tuberculosis in pleural fluid was compared by polymerase chain reaction (TB-DNA). The data were analyzed by SPSS 19.0 software. Results the positive rates of ESAT-6,CFP-10,E/C in the observation group were 85.4%, 75.0% and 91.7% respectively, which were significantly higher than those in the control group (P0.05). The sensitivity, Joden index and diagnostic accuracy of T-SPOT.TB were significantly higher than those of T-SPOT.TB (ESAT-6) and T-SPOT.TB (CFP-10). The sensitivity, specificity, Joden index and diagnostic accuracy of T-SPOT.TB were better than those of other methods, and the difference was statistically significant (P0.05). Conclusion the T cell dot test of Mycobacterium tuberculosis is sensitive, specific and accurate in the diagnosis of tuberculous pleurisy.
【作者单位】：青海省第四人民医院呼吸一科;青海省第四人民医院检验科;
【基金】：青海省卫生厅基金资助项目(QW-2010B043)
【分类号】：R52

【相似文献】