广西HIV-1流行株env基因C2V3区序列特征和亚型研究

发布时间：2019-05-12 17:36

【摘要】：目的研究广西艾滋病病毒1型流行株的亚型及env基因C2V3区序列变异特征。方法 2009-2012年共收集138份广西HIV-1感染者血浆样本,提取病毒RNA,用巢式聚合酶链反应对env基因C2V3区序列进行扩增,并测序,用MEGA、Hy Phy等软件对序列信息进行分析。结果 138份样本中,CRF01_AE亚型72例;CRF08_BC亚型40例;B亚型13例;CRF07_BC亚型8例;C亚型2例。各亚型基因离散率分别为:CRF01_AE(0.052±0.053);CRF08_BC(0.044±0.048);CRF07_BC(0.030±0.041);B亚型(0.024±0.044)。各亚型之间基因离散率差异无统计学意义(F=1.260,P=0.291)。CRF01_AE的负向选择压力比CRF08_BC大(t=10.060,P0.001)。共发现7种V3环顶端四肽,其中CRF01_AE有6种,CRF08_BC亚型有2种,两者均以GPGQ为主;B(B’)亚型有2种,以GPGR为主,CRF07_BC仅出现了1种。在CRF01_AE亚型和B亚型中分别发现了新的V3环顶端四肽GPGM和GTGR。各亚型辅助受体均主要为CCR5。结论 CRF01_AE亚型在广西HIV-1流行株中占优势,其V3环顶端四肽以GPGQ为主。CRF01_AE亚型和B亚型V3环序列出现了新的变异特征。广西HIV-1流行株主要为巨噬细胞嗜性的非合胞体诱导型。
[Abstract]:Objective to study the subtypes of HIV 1 epidemic strain and the sequence variation of C2V3 region of env gene in Guangxi. Methods A total of 138 plasma samples of HIV-1 infected people in Guangxi were collected from 2009 to 2012. The C2V3 region of env gene was amplified by nest polymerase chain reaction (NPCR) and sequenced. The sequence information was analyzed by MEGA,Hy Phy and other software. Results among the 138 samples, there were 72 cases of CRF01_AE subtypes, 40 cases of CRF08_BC subtypes, 13 cases of subtypes B, 8 cases of CRF07_BC subtypes and 2 cases of subtypes C. The gene dispersion rates of each subgroup were CRF01_AE (0.052 卤0.053), CRF08_BC (0.044 卤0.048) and CRF07_BC (0.030 卤0.041); B) (0.024 卤0.044), respectively. There was no significant difference in gene dispersion rate among subtypes (F 鈮，

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