青海省玉树州不同宿主间狂犬病病毒感染的分子流行病学分析
发布时间：2019-05-19 17:52
【摘要】：目的分析3株经野狼-家犬-牦牛传播的狂犬病病毒N基因部分核酸序列,以确定其传染源和传染途径。方法分离发病动物的RNA进行DFA检测和RT-PCR检测,阳性者进行N基因部分编码区核苷酸测序检测,采用ATCG ver4软件进行序列拼接,用DNAMAN将所测序列N基因进行核苷酸序列同源性比对,用ClustalX软件Bootstrap N-J Tree法构建系统发生树。结果发病宿主动物脑组织标本和脊髓经DFA检测到内基氏小体和病毒颗粒,RT-PCR扩增狂犬病毒核酸阳性,扩增产物大小为256bp,与目标片段大小一致。RT-PCR扩增产物经纯化后测序,N基因核苷酸序列3毒株间的同源性为99.28%~99.73%,推导的氨基酸序列同源性为99.46%~100.00%,牦牛狂犬病毒核苷酸序列与咬伤犬同源性99.28%,在自毙狼和病犬同源性为100%,牦牛狂犬病毒核苷酸序列与本室2012年测序的玛多犬N基因核苷酸同源性99.7%,与2012年内蒙古野生狸neimeng102B(EU652445.1)的同源性为95.28%,与2008年USA994-dog(EF611868.1)的同源性为95.94%,与2009BeijingHu1(EU700031.1)的同源性为85.9%。3株狂犬病毒株间N基因变异较小,稳定性较高,病毒核苷酸序列均为基因China IV,具有一定的地域性,进化分析显示均属于与野生动物最为相关的北极群。结论狂犬病可经野狼-家犬-家养牦牛传播,本起疫情中野狼为第一传染源。
[Abstract]:Objective to analyze the partial nucleic acid sequence of rabies virus N gene transmitted by wolf, domestic dog and yak, and to determine the source of infection and route of transmission. Methods the RNA of the diseased animals were detected by DFA and RT-PCR, the nucleotides in the partial coding region of N gene were sequenced, and the sequences were spliced by ATCG ver4 software. The nucleotide sequence homology of the N gene was compared by DNAMAN, and the phylogenetic tree was constructed by ClustalX software Bootstrap N 鈮，
本文编号：2480935
[Abstract]:Objective to analyze the partial nucleic acid sequence of rabies virus N gene transmitted by wolf, domestic dog and yak, and to determine the source of infection and route of transmission. Methods the RNA of the diseased animals were detected by DFA and RT-PCR, the nucleotides in the partial coding region of N gene were sequenced, and the sequences were spliced by ATCG ver4 software. The nucleotide sequence homology of the N gene was compared by DNAMAN, and the phylogenetic tree was constructed by ClustalX software Bootstrap N 鈮，
本文编号：2480935
本文链接：https://www.wllwen.com/yixuelunwen/chuanranbingxuelunwen/2480935.html
最近更新
教材专著
