日本斑点热立克次体近缘株重组OmpB蛋白抗原性研究
发布时间：2019-05-31 18:15
【摘要】：目的探讨关于斑点热立克次体的临床诊断及流行病学调查而进行的诊断方法学研究。方法构建R.Japonica Anhui 120 strain OmpB原核表达质粒,表达、鉴定并纯化重组蛋白,用其作为抗原建立间接ELISA检测方法,检测120份临床样本中日本斑点热立克次体特异性IgG抗体,并评价方法的敏感性和特异性。结果获得了高表达、高纯度的OmpB重组蛋白,建立的间接ELISA方法体系与美国食品和药物管理局认可的诊断试剂盒相比有良好的特异性和敏感性,两者的特异性为100%,敏感性为96.7%,符合率为99.2%,Kappa值为0.98。结论 OmpB重组蛋白具有很好的免疫反应性,能特异地检出斑点热立克次体IgG抗体,间接ELISA方法可作为临床诊断的技术储备及流行病调查和科学研究。
[Abstract]:Objective to investigate the clinical diagnosis and epidemiological investigation of Rickettsia speckle fever. Methods the prokaryotic expression plasmid R.Japonica Anhui 120 strain OmpB was constructed, the recombinant protein was identified and purified, and the indirect ELISA assay was established to detect the specific IgG antibody of Rickettsia macularis in 120 clinical samples. The sensitivity and specificity of the method were evaluated. Results High expression and high purity of OmpB recombinant protein were obtained. The indirect ELISA method system had good specificity and sensitivity compared with the diagnostic kit approved by the Food and Drug Administration of the United States, and the specificity of the two methods was 100%. The sensitivity was 96.7%, the coincidence rate was 99.2%, and the Kappa value was 0.98. Conclusion OmpB recombinant protein has good immunoreactivity and can detect IgG antibody against Rickettsia speckle fever in different places. Indirect ELISA method can be used as a technical reserve for clinical diagnosis, epidemiological investigation and scientific research.
【作者单位】： 安徽医科大学微生物教研室;
【基金】：国家自然科学基金(编号:81571963) 安徽省自然科学基金(编号:1608085MH213) 安徽省高等学校省级自然科学研究项目(编号:KJ2015A020、KJ2016A331) 安徽医科大学博士启动基金(编号:XJ201314、XJ201430、XJ201503)
【分类号】：R513
本文编号：2489921
[Abstract]:Objective to investigate the clinical diagnosis and epidemiological investigation of Rickettsia speckle fever. Methods the prokaryotic expression plasmid R.Japonica Anhui 120 strain OmpB was constructed, the recombinant protein was identified and purified, and the indirect ELISA assay was established to detect the specific IgG antibody of Rickettsia macularis in 120 clinical samples. The sensitivity and specificity of the method were evaluated. Results High expression and high purity of OmpB recombinant protein were obtained. The indirect ELISA method system had good specificity and sensitivity compared with the diagnostic kit approved by the Food and Drug Administration of the United States, and the specificity of the two methods was 100%. The sensitivity was 96.7%, the coincidence rate was 99.2%, and the Kappa value was 0.98. Conclusion OmpB recombinant protein has good immunoreactivity and can detect IgG antibody against Rickettsia speckle fever in different places. Indirect ELISA method can be used as a technical reserve for clinical diagnosis, epidemiological investigation and scientific research.
【作者单位】： 安徽医科大学微生物教研室;
【基金】：国家自然科学基金(编号:81571963) 安徽省自然科学基金(编号:1608085MH213) 安徽省高等学校省级自然科学研究项目(编号:KJ2015A020、KJ2016A331) 安徽医科大学博士启动基金(编号:XJ201314、XJ201430、XJ201503)
【分类号】：R513
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