放射线治疗小鼠泡球蚴病的超微结构观察

发布时间：2019-06-25 20:22

【摘要】：目的观察放射线治疗小鼠继发性泡球蚴病的疗效,探索新的非手术治疗泡球蚴病方法。方法筛选感染泡球蚴模型小鼠40只,随机分为5组,其中高能6Mev X射线组15只,6Mev能量电子线组5只,8Mev能量电子线组5只,60 Co-γ射线组5只,模型对照组10只(不作任何处理)。实验组小鼠行放射线治疗,5d后剖检,观察泡球蚴囊大体形态及超微结构变化并与模型对照组比较,评泡球蚴感染小鼠放射线治疗效果。结果与模型对照组相比较,6Mev Xray、6Mev E-ray、8Mev E-ray、60 Co-γray治疗组小鼠感染的泡球蚴囊壁组织和生发层细胞均有不同程度的改变和破坏,如线粒体肿胀、核固缩、糖原颗粒增多等,以高剂量组改变和破坏程度更显著。结论 6Mev X-ray、6Mev E-ray、8Mev E-ray、60 Co-γray治疗对小鼠泡球蚴的生长有一定的抑制作用,可使泡球蚴囊发生明显的形态学改变。
[Abstract]:Objective to observe the efficacy of radiation in the treatment of secondary alveolar echinococcosis in mice and to explore a new non-surgical method for the treatment of alveolar echinococcosis. Methods 40 mice infected with alveolar cercaria were randomly divided into 5 groups: high energy 6Mev X-ray group (n = 15), 6Mev energy electron ray group (n = 5), 8Mev energy electron line group (n = 5), 60 Co- gamma ray group (n = 5) and model control group (n = 10). The mice in the experimental group were treated with radiation therapy and examined 5 days later. The gross morphology and ultrastructure of alveolar echinococcosis cysts were observed and compared with the model control group to evaluate the effect of radiation therapy in mice infected with alveolar echinococcosis. Results compared with the model control group, the cysticercosis tissue and germatid cells of mice infected with 6Mev Xray,6Mev E / ray, 8Mev E / ray and 60 Co- 纬 ray groups changed and destroyed in varying degrees, such as swelling of mitochondria, pyknosis of nucleus and increase of glycogen granules, and the degree of change and destruction was more significant in the high dose group. Conclusion the treatment of 6Mev X / ray, 6Mev E / ray, 8Mev E / ray, 60 Co- 纬 ray can inhibit the growth of alveolar echinococcosis in mice, and can significantly change the morphology of alveolar echinococcosis cysts.
【作者单位】：甘肃中医学院病原生物学与免疫学教研室;兰州大学包虫病研究所;
【分类号】：R532.3

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