两种结核病病原菌快速检测技术的效果比较

发布时间：2019-07-21 10:15

【摘要】：目的探讨环介导等温扩增法与胶体金免疫层析法在结核病病原菌快速检测效果比较。方法选择2013年10月至2015年5月于我中心就诊的71例疑似结核病患者,分别取痰样本和血样本各71份,痰样本分别采用环介导等温扩增法和罗氏固体培养法进行病原菌检测。血样本采用胶体金免疫层析法进行病原菌检测。以罗氏固体培养法结果为参照,评价环介导等温扩增法和胶体金免疫层析法诊断的准确性。结果以罗氏固体培养法结果为参照,环介导等温扩增法的灵敏度为90.32%,特异度为97.50%,准确率为95.77%;胶体金免疫层析法灵敏度为97.10%,特异度为100%,准确率为94.37%。结论胶体金层析法和环介导等温扩增法均可获得较为满意的诊断结果,临床上可根据患者症状选择不同的诊断技术。
[Abstract]:Objective to compare the effect of ring-mediated isotherm amplification and colloidal gold immunochromatography in the rapid detection of tuberculosis pathogens. Methods from October 2013 to May 2015, 71 suspected tuberculosis patients were selected and 71 sputum samples and 71 blood samples were taken respectively. Sputum samples were detected by ring-mediated isotherm amplification and Roche solid culture respectively. Blood samples were detected by colloidal gold immunochromatography. Based on the results of Roche solid culture method, the diagnostic accuracy of ring-mediated isotherm amplification and colloidal gold immunochromatography was evaluated. Results compared with the results of Roche solid culture method, the sensitivity, specificity and accuracy of ring-mediated isotherm amplification were 90. 32%, 97. 50% and 95. 77%, respectively, and the sensitivity, specificity and accuracy of colloidal gold immunochromatography were 97. 10%, 100% and 94. 37% respectively. Conclusion Colloidal gold chromatography and ring-mediated isotherm amplification can obtain satisfactory diagnostic results, and different diagnostic techniques can be selected according to the symptoms of the patients.
【作者单位】： 大庆市疾病预防控制中心;
【分类号】：R52

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