HBV母婴传播的病毒学特征及脐带血和外周血的诊断价值
发布时间：2021-08-06 13:41
背景及目的:HBV母婴传播有三种可能的传播方式:宫内传播,分娩时传播和产后传播。实施联合免疫后,大多数研究认为联合免疫失败的婴儿可能发生了宫内感染。一些学者用新生儿出生时HBV-M特征分析HBV母婴传播。有些研究认为脐带血安全,操作简单,家属易接受,所以用脐带血HBV-M分析母婴传播。也有研究认为脐带血容易受到母血的污染,故新生儿出生时外周血分析HBV母婴传播。我们的研究同时采集新生儿脐带血和外周血。重点是对HBsAg阳性孕妇分娩的新生儿HBV特征进行分析,同时对新生儿外周血和脐带血的HBV标记物进行定量的检测和比较分析,通过其消长和变化的随访分析,进一步探讨其作为HBV母婴传播早期诊断的价值。方法:招募深圳市第三人民医院2011-2016年期间分娩的750例慢性HBV感染的孕妇及其新生儿为研究对象。本试验对386例签署知情同意书的HBsAg阳性的孕妇留取分娩前外周血及其新生儿脐带血和外周血;对新生儿随访至7-12个月,进行乙肝病毒血清学标记物和HBV DNA的检测。本研究应用荧光定量PCR检测HBV DNA,同时采用化学发光法定量检测HBsAg、HBsAb、HBeAg、HBeAb、H...
【文章来源】：南华大学湖南省
【文章页数】：73 页
【学位级别】：硕士
【部分图文】：
技术路线图
17图 2. 研究对象招募及随访过程Figure 2. Enrollment and foiiow-up of the Trial Participants.
图 3-1. HBsAg 阳性孕妇的新生儿脐带血 HBV 血清学特征Figure 3-1. Positivity rates of HBV virologicalmarkers in neonates (n = 386) born toHBsAg(+) mothers.Black columns represent those from the UC.
【参考文献】：
期刊论文
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本文编号：3325879
【文章来源】：南华大学湖南省
【文章页数】：73 页
【学位级别】：硕士
【部分图文】：
技术路线图
17图 2. 研究对象招募及随访过程Figure 2. Enrollment and foiiow-up of the Trial Participants.
图 3-1. HBsAg 阳性孕妇的新生儿脐带血 HBV 血清学特征Figure 3-1. Positivity rates of HBV virologicalmarkers in neonates (n = 386) born toHBsAg(+) mothers.Black columns represent those from the UC.
【参考文献】：
期刊论文
[1]深圳市居民乙型肝炎病毒感染现状及基因型分析[J]. 阳帆,路滟,张仁利,黄达娜,李玥,吴春利. 中国卫生检验杂志. 2015(15)
[2]乙型肝炎病毒感染女性生育管理专家共识[J]. 易为,胡玉红,刘敏. 中华实验和临床感染病杂志(电子版). 2014(01)
[3]我国孕妇乙肝表面抗原阳性率的Meta分析[J]. 石果,张顺祥. 中国预防医学杂志. 2013(01)
[4]Relationship of hepatitis B virus infection of placental barrier and hepatitis B virus intra-uterine transmission mechanism[J]. Han Bai,Lin Zhang,Li Ma,Xiao-Guang Dou,Guo-He Feng,Gui-Zhen Zhao,Department of Infectious Diseases,Shengjing Hospital Affiliated to CMU,Shenyang 110004,Liaoning Province,China. World Journal of Gastroenterology. 2007(26)
[5]Mechanism of intrauterine infection of hepatitis B virus[J]. Shu-Lin Zhang,Department of Infectious Disease,First Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,ChinaYa-Fei Yue Gui-Qin Bai Lei Shi,Department of Gynecology and Obstetrics,First Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,ChinaHui Jiang,Hospital of Women’s and Children’s Health Care,Zhaoqing 526060,Guangdong Province,China. World Journal of Gastroenterology. 2004(03)
[6]Interruption of HBV intrauterine transmission:A clinical study[J]. Xiao-Mao Li Yue-Bo Yang Hong-Ying Hou Zhong-Jie Shi Hui-Min Shen Ben-Qi Teng Ai-Min Li Ling Zou Department of Obstetrics and Gynecology,The Third Affiliated Hospital,Sun Yat-Sen University,Guangzhou 510630,Guangdong Province,China Min-Feng Shi Maternity Hospital,School of Medicine,Zhejiang University,Hangzhou 310006,Zhejiang Province,China. World Journal of Gastroenterology. 2003(07)
本文编号：3325879
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