CRISPR/Cas9-based tools for targeted genome editing and repl

发布时间：2023-09-02 12:34

　　Hepatitis B virus(HBV) infection remains a major global health problem because current therapies rarely eliminate HBV infections to achieve a complete cure. A different treatment paradigm to effectively clear HBV infection and eradicate latent viral reservoirs is urgently required. In recent years, the development of a new RNA-guided gene-editing tool, the CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9) system, has greatly facilitated site-spec...

【文章页数】：9 页

【文章目录】：
INTRODUCTION
HBV LIFE CYCLE
CRISPR/CAS9: A SIMPLE AND EFFICIENT TOOL FOR GENOME EDITING
MODULATION OF HBV REPLICATION WITH THE CRISPR/CAS9 SYSTEM
    Various HBV-specific sg RNA design regions and suppression efficiencies
    CRISPR/Cas9-mediated mutation of the HBV genome
    Combined application of CRISPR/Cas9 with other antiviral treatment
LIMITATIONS AND PROSPECTS
    Improve efficiency of the delivery system
    Avoidance of off-target effects
    Optimization of target sites
CONCLUSION
COMPLIANCE WITH ETHICS GUIDELINES



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