山麻鸭开产期和产蛋高峰期卵巢组织转录组分析

发布时间：2017-12-28 03:04

本文关键词：山麻鸭开产期和产蛋高峰期卵巢组织转录组分析　出处：《中国农业科学》2016年05期 　论文类型：期刊论文

【摘要】：【目的】探明山麻鸭开产期和产蛋高峰期卵巢组织的转录组差异,丰富蛋鸭转录组数据信息。【方法】选取6份山麻鸭卵巢组织样品(开产期和产蛋高峰期各3份),利用Illumina Hi SeqTM 2000进行高通量测序,构建山麻鸭开产期和产蛋高峰期卵巢组织转录组文库,并用测序评估、基因功能注释等生物信息学方法进行分析。【结果】经过测序获得质量不低于20的碱基比例(Q20)均高于93%;开产期获得了43 489 798条reads,4 380 847 061 bp数据量;高峰期获得了42 782 676条reads,4 307 499 083 bp数据量;分别有70.92%和72.70%的reads能比对到鸭参考基因组序列上。对开产期与产蛋高峰期转录组进行比较,发现开产期有26 808个表达基因,产蛋高峰期有27 013个表达基因,与开产期为参考,在高峰期卵巢组织中共获得1 929个差异表达基因,其中上调基因989个,下调基因940个;进一步将这些差异基因在Nr数据库进行注释,发现获得注释的基因有1 423个,其中上调基因695个,下调基因728个;COG功能注释分析发现这些差异表达基因共获得663个功能注释,涉及25个功能分类;GO功能注释分析表明,有1 122个基因获得GO功能注释,可以分为61个功能分类,这些分类主要涉及到分子绑定、催化活性、细胞过程、生物调节等诸多生理生化过程,其中涉及到发育繁殖生物学过程的相关注释基因有406个;KEGG分析发现共有425个基因注释到160个代谢通路中,其中Gn RH信号通路、GPI-anchor生物合成、TGF-β信号通路、核糖体生物合成等通路显著富集。Gn RH信号通路和TGF-β信号通路在卵巢的生理生殖活动发挥了重要作用,而GPI-anchor生物合成和核糖体生物合成,这两类代谢通路在卵巢生理生殖活动的作用尚不明确。【结论】利用高通量测序技术对山麻鸭开产期和产蛋高峰期卵巢组织的转录组进行测序和分析,揭示了山麻鸭不同生理状态下卵巢组织差异表达基因的数量,获得了差异表达基因的功能、分类和代谢通路。为丰富蛋鸭卵巢组织转录组信息,为开展蛋鸭产蛋性状相关基因的研究及分子调控机制研究奠定基础。
[Abstract]:[Objective] the transcriptome differences in perinatal period and peak period of laying open mountain duck ovary, rich duck transcriptome data. [Methods] a total of 6 cases of ovarian tissue samples (open ShanMa duck egg peak in perinatal period and 3 copies each), using Illumina Hi SeqTM 2000 high-throughput sequencing, construct ShanMa duck egg peak period and open ovarian tissue transcriptome library and sequencing evaluation, gene annotation and other bioinformatics analysis. [results] after sequencing was not less than 20 of the mass base ratio (Q20) were higher than 93%; opening date obtained 43489798 reads and 4380847061 BP data; the peak obtained 42782676 reads and 4307499083 BP data; there were 70.92% and 72.70% reads compared to the reference genome sequence of duck. Comparison of open perinatal and egg peak transcriptome, found 26808 gene expression in perinatal period, egg laying peak period of 27013 gene expression, and open period for reference, at the peak of the ovarian tissue were obtained 1929 differentially expressed genes, including 989 up-regulated genes and 940 down regulated genes; these will further differential gene annotation in the Nr database, find notes 1423 genes, including 695 up-regulated genes and 728 down regulated genes; COG functional annotation analysis of gene expression were obtained 663 functional annotation of these differences, involving 25 functional classification; GO functional annotation analysis showed that 1122 genes for GO annotation can be divided 61 functional classification, the classification is mainly related to the molecular binding, catalytic activity, cellular process, biological regulation and other physiological and biochemical process, which involves the development of reproductive biology There were 406 annotated genes in the process. KEGG analysis revealed that 425 genes were annotated to 160 metabolic pathways, of which Gn RH signaling pathway, GPI-anchor biosynthesis, TGF- beta signaling pathway and ribosome biosynthesis pathway were significantly enriched. Gn RH signaling pathway and TGF- beta signaling pathway play an important role in the physiological and reproductive activities of the ovary, while GPI-anchor biosynthesis and ribosome biosynthesis, these two kinds of metabolic pathways are not clear in the physiological and reproductive activities of the ovary. [Conclusion] the transcriptome using high-throughput sequencing technology in perinatal period and egg peak of ShanMa duck ovarian tissues were sequenced and analyzed, revealed the differences in ovarian tissue ShanMa duck under different physiological conditions of gene expression quantity, function, classification and the differences of metabolic pathways of gene expression. Rich duck ovarian tissue transcriptome information for research and molecular gene related traits of laying ducks lay the foundation for study on the mechanism of regulation.
【作者单位】：福建省农业科学院畜牧兽医研究所;龙岩市红龙禽业有限公司;
【基金】：福建省科技计划重大专项(2012NZ01040039) 福建省种业创新项目(2014S1477-13)
【分类号】：S834
【正文快照】： Transcriptome Analysis of Ovary Tissue in Early Laying Periodand Egg Laying Peak Period of Shanma DucksZHU Zhi-ming1,CHEN Hong-ping2,LIN Ru-long2,MIAO Zhong-wei1,XIN Qing-wu1,LI Li1,0引言【研究意义】提高家禽产蛋性能是家禽遗传改良的重要任务。产蛋性状属低

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