定量蛋白质组学技术分析猪繁殖与呼吸综合征病毒感染肺泡巨噬细胞的差异表达蛋白质

发布时间：2018-01-03 02:37

本文关键词：定量蛋白质组学技术分析猪繁殖与呼吸综合征病毒感染肺泡巨噬细胞的差异表达蛋白质　出处：《畜牧兽医学报》2016年06期 　论文类型：期刊论文

【摘要】：作者采用定量蛋白质组学方法,对猪繁殖与呼吸综合征病毒(PRRSV)感染猪肺泡巨噬细胞(PAMs)和正常PAMs的蛋白质组进行差异分析。通过高效液相色谱和电喷雾串联质谱仪定量分析PRRSV感染细胞与未感染对照细胞间的差异表达蛋白质,并运用Max-Quant 1.0.7.4软件结合蛋白质数据库PaxDb进行鉴定。与对照组相比,感染组共鉴定出39个差异表达蛋白质,其中30个蛋白质表达上调,9个蛋白质表达下调。通过Western blot和ELISA验证了感染的PAMs中SPP1、IFIT3、STAT3及IL-8的表达情况与质谱鉴定结果一致。通过液相色谱—串联质谱联用(LC-MS/MS)技术,筛选得到多个与PRRSV感染相关的差异表达蛋白质,为PRRSV发病的分子机制分析提供了新依据。
[Abstract]:The author adopts quantitative proteomics method, syndrome virus of porcine reproductive and respiratory infection (PRRSV) in porcine alveolar macrophages (PAMs) protein group and normal PAMs variance analysis. And electrospray tandem mass spectrometry quantitative analysis of PRRSV infected cells and uninfected cells differentially expressed proteins between control by HPLC, and the use of Max-Quant 1.0.7.4 software combined with the protein database of PaxDb were identified. Compared with the control group, infection group identified 39 differentially expressed proteins, including 30 proteins up-regulated and 9 proteins down regulated by Western. Blot and ELISA confirmed SPP1 infection in PAMs, IFIT3, STAT3 and IL-8 expression and mass spectrometry results consistent. Identification of tandem mass spectrometry by liquid chromatography (LC-MS/MS) technology, screened multiple related differences in PRRSV infection and protein expression for the pathogenesis of PRRSV The analysis of molecular mechanism provides a new basis.

【作者单位】：西北农林科技大学生命科学学院;
【基金】：中央高校基本科研业务费创新重点项目(QN2011065)
【分类号】：S858.28
【正文快照】： 猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)俗称蓝耳病,是由猪繁殖与呼吸综合征病毒(porcine reproductive andrespiratory syndrome virus,PRRSV)引起的一种高度传染性疾病,其主要特征是引起妊娠母猪严重的繁殖障碍和仔猪呼吸困难及高死亡率[1

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