不同生长时期梅花鹿鹿茸差异蛋白质组学分析

发布时间：2018-01-08 00:09

本文关键词：不同生长时期梅花鹿鹿茸差异蛋白质组学分析　出处：《畜牧兽医学报》2016年03期 　论文类型：期刊论文

【摘要】：旨在从分子水平认识鹿茸生长机制,本研究以10、40、60与130d的梅花鹿鹿茸为试验材料,运用双向凝胶电泳(2-DE)、质谱鉴定技术与生物信息学方法对梅花鹿鹿茸生长过程中差异表达蛋白质进行研究。结果显示,有46种蛋白质差异性表达,且主要参与细胞骨架、转运过程、信号转导、细胞凋亡、骨发育、蛋白质合成、核酸代谢、免疫、能量代谢、细胞增殖、抗氧化、蛋白质折叠等生物学过程。结合鹿茸的快速生长与快速骨化的独特生长过程,对差异表达蛋白质中的骨发育相关蛋白、抗氧化蛋白、细胞凋亡相关蛋白做进一步分析,发现P4HB、SPARC、过氧化物还原酶2、过氧化物还原酶4、半乳糖凝集素1、视黄酸结合蛋白1等6种蛋白质在鹿茸快速生长与快速骨化过程中起着重要的作用,为鹿茸生长与骨化机制的进一步研究奠定基础。
[Abstract]:In order to understand the growth mechanism of velvet antler at the molecular level, the two dimensional gel electrophoresis (2-DEE) was used to study the mechanism of velvet antler growth. The differential expression of proteins in the growth process of deer antler from sika deer was studied by mass spectrometry and bioinformatics. The results showed that 46 proteins were differentially expressed and were mainly involved in cytoskeleton and transport. Signal transduction, apoptosis, bone development, protein synthesis, nucleic acid metabolism, immunity, energy metabolism, cell proliferation, antioxidant. Protein folding and other biological processes. Combined with the rapid growth of velvet antler and rapid ossification of the unique growth process, differentially expressed proteins in bone development related proteins, antioxidant proteins. Further analysis of apoptosis-related proteins showed that P4HBSPARC, peroxidase 2, peroxidase 4, galactose agglutinin 1 were found. Six proteins, such as retinoic acid binding protein 1, play an important role in the rapid growth and rapid ossification of velvet antler, which lays a foundation for further study on the mechanism of antler growth and ossification.
【作者单位】：中国农业科学院特产研究所特种经济动物分子生物学重点实验室;
【基金】：特种动物遗传资源创新团队(CAAS-ASTIP-201X-ISAPS) 特种动物种质资源平台
【分类号】：S825
【正文快照】： 鹿茸是唯一完全再生的哺乳动物器官,并具有独特的生长过程。每年春天鹿角自动脱落,并从角柄上长出新鹿茸,随后快速生长并形成分支,秋天鹿茸的生长速度变慢,软骨组织逐渐骨化形成硬骨,次年春天再次脱落,开始新一轮的循环。鹿茸的生长可分为两个阶段:生长期与骨化期。在生长期,

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