高氨刺激下参与免疫应答和肌肉收缩过程的肉鸡气管蛋白差异表达——基于iTRAQ标记技术的差异蛋白质组学研究

发布时间：2018-01-18 01:19

本文关键词：高氨刺激下参与免疫应答和肌肉收缩过程的肉鸡气管蛋白差异表达——基于iTRAQ标记技术的差异蛋白质组学研究　出处：《中国科学:生命科学》2016年11期 　论文类型：期刊论文

【摘要】：氨气是造成家禽呼吸道疾病的主要污染气体,它能引起气管损伤,而该损伤机理还未见详细报道.因此,本研究采用同位素标记相对和绝对定量(i TRAQ)技术,研究高浓度氨气刺激下艾拔益加肉鸡(Gallus domesticus)(简称AA肉鸡)气管的蛋白质表达变化,以揭示氨气对气管损伤的潜在机理.本研究共鉴定3706个蛋白(FDR1%),其中119个蛋白差异表达.通过GO功能分析发现,参与免疫应答和肌肉收缩的蛋白显著富集.在免疫应答类别中,上调蛋白(FGA等)与促炎效应相关,而下调蛋白参与抗原处理和提呈(MYO1G等)、免疫球蛋白和抗菌肽的生成(fowlicidin-2等)、以及免疫缺陷(PTPRC等)过程.同时在肌肉收缩过程发挥重要作用的蛋白(TPM1等)均表达上调.作为呼吸道疾病的共同特征之一,本研究也发现了黏蛋白的高表达.最后,通过实时RT-PCR对6个差异蛋白的表达水平进行验证.研究结果不仅提示高氨刺激可能造成气管阻塞、降低宿主防御功能,也为将来抵御氨气毒性的措施提供了线索.
[Abstract]:Ammonia is the main pollutant gas causing respiratory diseases in poultry. It can cause trachea injury, and the mechanism of this injury has not been reported in detail. In this study, relative and absolute quantitative TRAQ techniques were used. To study the changes of protein expression in trachea of Gallus domesticusus (AA broiler) stimulated by high concentration ammonia. In order to reveal the potential mechanism of ammonia on trachea injury, a total of 3706 proteins were identified in this study, among which 119 proteins were differentially expressed. The protein involved in immune response and muscle contraction was significantly enriched. In the immune response category, the up-regulated protein FGA was associated with the pro-inflammatory effect, while the down-regulated protein was involved in antigen processing and presentation of MYO1G). The production of immunoglobulin and antimicrobial peptides, such as fowlicidin-2. The expression of TPM1, which plays an important role in muscle contraction, is up-regulated, which is one of the common features of respiratory diseases. This study also found the high expression of mucin. Finally, the expression level of 6 differentially expressed proteins was verified by real-time RT-PCR. The results not only suggested that high ammonia stimulation may cause tracheal obstruction. The reduction of host defense function also provides clues for future measures against ammonia toxicity.
【作者单位】：中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室;
【基金】：国家科技支撑计划(批准号:2012BAD39B02) 中国农业科学院创新工程项目(批准号:ASTIP-IAS07) 北京畜牧兽医研究所基本科研业务费项目(批准号:2015YWF-ZD-5)资助
【分类号】：S858.31
【正文快照】： 氨气是一种无色、有刺激性气体,被认为是家禽养殖中的主要污染物.在一定温度湿度条件下,氨气由微生物分解动物的排泄物而产生.虽然氨气的推荐浓度在25 m L/m3以内[1],但是由于大规模养殖下对环境的不当控制,禽类常常暴露在高浓度的氨气条件下,特别是在通风设施受限的冬季,情况

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