IGF-I及其受体IGF-IR基因在鸡胚胎腿肌发育过程中表达模式分析

发布时间：2018-01-18 15:30

本文关键词：IGF-I及其受体IGF-IR基因在鸡胚胎腿肌发育过程中表达模式分析　出处：《中国农业大学学报》2017年01期 　论文类型：期刊论文

更多相关文章：鸡 IGF-I IGF-IR 腿肌胚胎 mRNA

【摘要】：为研究胰岛素样生长因子IGF-I及其受体IGF-IR在鸡胚胎腿部肌肉发育过程中的作用,选取藏鸡和矮小蛋鸡受精蛋孵化后,采集鸡胚胎孵化第9天、第13天和第20天时腿部肌肉组织,并利用荧光定量PCR技术检测IGF-I和IGF-IR mRNA的表达水平,同时检测IGF-I基因编码区的单核苷酸多态性并利用RFLP(限制性片段长度多态性)方法对筛选到的单核苷酸多态性进行群体水平分型。结果表明:藏鸡和矮小蛋鸡胚胎腿部肌肉组织在孵化第9天和第13天时的IGF-I和IGF-IR的mRNA表达量均极显著高于第20天,且藏鸡IGF-I的mRNA表达量在第9天也显著高于第13天,而矮小蛋鸡的IGF-I mRNA表达水平在第9天和第13天无明显差异。此外,在整个孵化过程中,矮小蛋鸡的IGF-IR的表达模式与IGF-I表达模式基本一致。而藏鸡IGF-IR的表达水平在第9天明显高于第13天但未达到显著水平(P0.05),同时发现第9天藏鸡IGF-IR的表达水平也高于矮小蛋鸡(P0.05)。除此之外,在藏鸡和矮小蛋鸡的IGF-I的编码区均发现c.282AG单核苷酸多态性,该SNP在藏鸡中主要以杂合子AG基因型为优势基因型,基因型频率为0.57,而在矮小蛋鸡中主要以GG基因型为优势基因型,基因型频率为0.77。根据试验结果分析得出,IGF-I和IGF-IR在腿部肌肉组织的表达水平在鸡胚胎孵化中期(第9天和第13天)显著高于孵化后期(第20天),可能是由于中期属于胚胎腿部肌肉快速发育阶段而后期腿部肌肉发育变得相对迟缓,同时IGF-I和IGF-IR的表达水平也随之发生变化。
[Abstract]:In order to study the role of insulin-like growth factor (IGF-I) and its receptor IGF-IR in the muscle development of the legs of chicken embryos, Tibetan and dwarf laying eggs were selected and hatched on the 9th day after hatching. The expression levels of IGF-I and IGF-IR mRNA were detected by fluorescence quantitative PCR on the 13th and 20th day. Single nucleotide polymorphisms in the coding region of IGF-I gene were also detected and RFLP (restriction fragment length polymorphism) was used. Methods the selected single nucleotide polymorphisms were classified at population level. The mRNA expression of IGF-I and IGF-IR in the leg muscle of Tibetan chicken and dwarf laying hens on the 9th and 13th day of hatching was significantly higher than that on the 20th day. The mRNA expression of IGF-I in Tibetan chickens was also significantly higher on the 9th day than on the 13th day, but there was no significant difference in the IGF-I mRNA expression level of the dwarf layers on the 9th and 13th days. In addition, there was no significant difference in the expression of IGF-I mRNA between the two groups on the 9th day and the 13th day. Throughout the incubation process. The expression pattern of IGF-IR in dwarf layers was basically consistent with that of IGF-I, while the expression of IGF-IR in Tibetan chickens was significantly higher than that in day 13 on the 9th day (P < 0.05). P0.05). At the same time, it was found that the expression level of IGF-IR in Tibetan chickens on the 9th day was also higher than that in dwarf layers. The single nucleotide polymorphism of c.282 AG was found in the coding region of IGF-I of Tibetan and dwarf layers, and the dominant genotype of SNP was heterozygous AG genotype in Tibetan chickens. The genotype frequency was 0.57, while the GG genotype was the dominant genotype and the genotype frequency was 0.77 in the dwarf laying hens. The expression levels of IGF-I and IGF-IR in the muscle tissue of the legs were significantly higher than those in the late hatching period (day 9 and day 13). This may be due to the rapid development of the leg muscles in the middle stage of the embryo and the relatively slow development of the leg muscles in the later stages. Meanwhile, the expression levels of IGF-I and IGF-IR also change.
【作者单位】：西藏农牧学院动物科学学院;中国农业大学动物科学技术学院;
【基金】：国家科技支撑项目(2012BAD03B03) 西藏特色农牧资源研发协同创新中心——藏猪项目(XZ2014006) 西藏特色畜牧种质资源创新平台项目(XZ2015005)
【分类号】：S831
【正文快照】： 在哺乳动物中,生长激素可以促进动物的生长发育。然而对于鸡,高生长激素个体往往表现出较慢的生长速度[1-2]。这说明鸡生长激素不能直接影响其生长速度,需要一些下游的因子进行辅助,这些因子就是胰岛素样生长因子。胰岛素生长因子(Insulin-like growth factors,IGFs)在脊椎动

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