薄荷油对雌性布氏田鼠的抗生育效果

发布时间：2018-01-18 16:00

本文关键词：薄荷油对雌性布氏田鼠的抗生育效果　出处：《四川动物》2017年03期 　论文类型：期刊论文

更多相关文章： 布氏田鼠 薄荷油 胚胎着床 抗生育

【摘要】：我国草地鼠害发生面积呈逐年扩增的趋势。布氏田鼠Lasiopodomys brandtii是内蒙古草原主要害鼠之一,为找到有效控制鼠类数量的方法,本文研究了薄荷油对布氏田鼠的抗生育效果。以性成熟的非越冬布氏田鼠为实验对象,将备用的雌、雄布氏田鼠按2∶1合笼,将妊娠布氏田鼠分为3个不同药物处理组和1个空白对照组,将薄荷油用0.5%羧甲基纤维素钠配置成不同浓度的溶液(750 mg·kg~(-1)、1 000 mg·kg~(-1)、1 500 mg·kg~(-1)),灌胃7 d后剖检,统计其子宫胚胎着床数,对子宫进行组织病理学分析。实验结果表明,薄荷油能有效降低布氏田鼠胚胎着床数(P0.01)。扫描电子显微镜结果表明,薄荷油使布氏田鼠子宫内膜表面容受性发生了改变;组织病理学结果表明,经薄荷油处理的布氏田鼠子宫形态发生了改变,产生病变;免疫组织化学实验结果显示,药物处理组子宫内的雌激素受体(ERα)、孕激素受体(PR)、基质金属蛋白水解酶(MMP2)及E-钙粘附蛋白(E-cadherin)等相关蛋白的表达水平都出现了异常。这些研究结果表明,薄荷油对布氏田鼠的抗生育作用主要是通过影响调节子宫内ERα、PR、MMP2及E-cadherin等相关蛋白的表达水平来影响其子宫内膜容受性及胚胎着床过程,进而导致胚胎着床失败。因此,薄荷油可作为一种潜在的植物源性不育剂应用于鼠类不育控制。
[Abstract]:The area of rodent damage in grassland of China is increasing year by year. Lasiopodomys brandtii is one of the main pests in Inner Mongolia grassland. In order to find an effective method to control the number of rodents, the antifertility effect of menthol on brucelian voles was studied. Male brucovar voles were divided into three different drug treatment groups and a blank control group by 2: 1 cage. The peppermint oil was prepared with 0.5% sodium carboxymethyl cellulose into different concentration solutions of 750mg 路kg ~ (-1) ~ (-1) ~ (1 000 mg 路kg ~ (-1)). 1 500 mg 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1) 路L ~ (-1), after 7 days of gastric perfusion, the number of uterus embryo implantation was counted, and the histopathological analysis of the uterus was carried out. The results showed that: 1. The results of scanning electron microscope showed that menthol could change the receptivity of brucellitic vole's endometrium. The histopathological results showed that the uterine morphology of brucellitic voles treated with menthol was changed and pathological changes occurred. The results of immunohistochemistry showed that estrogen receptor ER 伪 and progesterone receptor PRA were detected in the uterus of the drug treated group. The expression levels of matrix metalloproteinase (MMP2) and E-cadherin (E-cadherin) were abnormal. The antifertility effect of menthol on brucelian voles is mainly through the regulation of ER 伪 -PR in uterus. The expression level of MMP2 and E-cadherin may affect the endometrial receptivity and embryo implantation process, which leads to the failure of embryo implantation. Menthol can be used as a potential plant sterility agent to control sterility in mice.
【作者单位】：四川大学生命科学学院;
【基金】：公益性行业(农业)科研专项项目(201203041) 四川省科技支撑计划项目(2012SZ0045)
【分类号】：S812.6
【正文快照】： 草地是我国重要的农牧业自然资源和重要的生态屏障,鼠害是草地开发利用过程中出现的严重生态学问题(李贺新,2011)。我国草地鼠害发生面积呈逐年扩增的趋势,鼠害造成的牧草损失超过60亿元(洪军等,2014)。鼠害的大规模发生会导致草场土壤肥力下降、水分流失、草地生产力降低、杂

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