两种复合植物提取物对断奶仔猪生产性能、血液生化及抗氧化功能的影响

发布时间：2018-01-19 00:16

本文关键词： 植物提取物断奶仔猪生长性能血液生化指标抗氧化　出处：《南京农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：由于耐药性与残留问题,欧洲各国从上世纪九十年代开始逐渐禁止抗生素作为生长促进剂添加到畜禽饲粮中。在此背景下,植物提取物被认为是极具有潜能的抗生素生长促进剂的替代品。本研究通过体外试验探讨两种复合植物提取Fresta-F和Aromex-ME的体外抗氧化能力和在断奶仔猪日粮中分别添加 Fresta-F和Aromex-ME,探讨其对断奶仔猪生产性能、血液生化指标、血清抗氧化指标的影响,为植物提取物在动物生产中的应用提供试验依据。本文共包括三个试验:试验一,研究两种复合植物提取物Fresta-F和Aromex-ME对断奶仔猪生产性能和饲料养分利用率的影响。本试验选择胎次、体重接近以及遗传基础一致,公母各半的180头,24日龄(杜×长×大)断奶仔猪为试验对象,随机分为3组,每组再随机分6个重复(每个重复10头,公母各半)。对照组饲喂基础日粮,两个试验组在基础日粮的基础上分别添加250mg/kg的Fresta-F和100mg/kg的Aromex-ME。试验期40天。试验结果表明:(1)实验结束时,两个试验组的料重比较对照组分别降低13.13%(P0.05)和9.38%(P0.05);平均日增重较对照组分别提高12.21%(P0.05)和10.78%(P0.05)。(2)两种植物提取物显著提高了日粮中干物质利用率(P0.05)和粗蛋白利用率(P0.05)。(3)两种植物提取物对断奶仔猪的腹泻率和死亡率均没有显著影响(P0.05)。试验二,研究两种复合植物提取物Fresta-F和Aromex-ME对断奶仔猪血液生化和血清脂代谢的影响。试验分组和试验期限同试验一。试验结果表明:(1)试验中期(44d)时,植物提取物Fresta-F和Aromex-ME能显著降低血清中TP含量,下降幅度分别达到19.27%(P0.05)和17.13%(P0.05),同时Aromex-ME能显著降低血清中UN的含量,下降幅度达25.06%(P0.05)。但在试验中期和后期,对血液生化其他的指标均无显著影响(P0.05)。(2)植物提取物Fresta-F和Aromex-ME能显著降低试验中期(44d)、试验结束时(64d)时仔猪血清中TC含量。试验结束时(64d),两个试验组的HDL-C均显著上升,上升幅度分别为23.29%(P0.05)和26.03%(P0.05),同时植物提取物Fresta-F组的LDL-C含量显著下降,下降幅度达到33.57%(P0.05)。试验三,研究两种复合植物提取物Fresta-F和Aromex-ME对断奶仔猪血清抗氧化指标的影响和其体外抗氧化能力。体外抗氧化能力测试包括对DPPH和ABTS自由基清除作用以及其总抗氧化能力评价(FRAP)。试验结果表明:两种复合植物提取物Fresta-F和Aromex-ME对试验中期(44d)血清总抗氧化能力、丙二醛水平、超氧化物歧化酶活性具有明显的改善作用(P0.05),但对于试验后期(64d),改善作用不显著(P0.05);两种复合植物提取物Fresta-F和Aromex-ME具有一定的清除自由基能力和还原力,但都低于BHT。综上所述,植物提取物Fresta-F和Aromex-ME可以提高断奶仔猪的生产性能、降低血脂以及改善早期断奶仔猪的血清抗氧化能力。
[Abstract]:Due to drug resistance and residual problems, European countries have gradually banned antibiotics as growth promoters in livestock and poultry diets since -10s. Plant extracts are considered to be the most potent substitutes for antibiotic growth promoters. In this study, the in vitro oxygen resistance of Fresta-F and Aromex-ME extracted from two complex plants was investigated in vitro. Chemical ability and supplementation in weaning piglets' diets. Fresta-F and Aromex-ME. In order to provide experimental basis for the application of plant extract in animal production, the effects on weaning piglets' production performance, blood biochemical index and serum antioxidant index were studied. The effects of two compound plant extracts (Fresta-F and Aromex-ME) on the performance and feed nutrient utilization of weaned piglets were studied. The body weight was close and the genetic basis was the same. 180 weaned piglets of 24 days old (du 脳 long 脳 big) were randomly divided into 3 groups and each group was randomly divided into 6 replicates (10 replicates each). The control group was fed with basal diet. The two groups were supplemented with 250 mg / kg Fresta-F and 100 mg / kg Aromex-ME.The trial period was 40 days. 1) at the end of the experiment. Compared with the control group, the feed weight of the two groups was decreased by 13.13% (P 0.05) and 9.38% (P 0.05) respectively. Compared with the control group, the average daily gain increased by 12.21% (P 0.05) and 10.78% (P 0.05), respectively. P0.05) and crude protein utilization ratio (P0.05) were not significantly affected by two plant extracts on diarrhea rate and mortality of weaned piglets. Experiment 2. The effects of two compound plant extracts Fresta-F and Aromex-ME on blood biochemistry and serum lipid metabolism in weaned piglets were studied. 1) the middle period of the test was 44 days. Plant extract Fresta-F and Aromex-ME could significantly reduce the content of TP in serum. The decrease was as high as 19.27% (P 0.05) and 17.13% P 0.05 (P 0.05), respectively. Meanwhile, Aromex-ME could significantly reduce the content of UN in serum. The decrease was up to 25.06% (P 0.05), but in the middle and late stages of the experiment. There was no significant effect on other biochemical indexes of blood (P0.05. 0. 0. 0. 0. 0. 0. 0. 0. 2) Fresta-F and Aromex-ME of plant extracts decreased significantly in the middle period of the experiment for 44 days). At the end of the experiment, the serum TC content of the piglets and the HDL-C of the two groups increased significantly at the end of the experiment. The rising ranges were 23.29m (P 0.05) and 26.03g (P 0.05), respectively. Meanwhile, the LDL-C content in the Fresta-F group was significantly lower than that in the control group. The range of decrease was 33.57% (P 0.05). Experiment 3. To study the effects of two compound plant extracts Fresta-F and Aromex-ME on serum antioxidant indexes of weaned piglets and their antioxidant capacity in vitro. The in vitro antioxidant capacity test included DPPH. And ABTS free radical scavenging effect and its total antioxidant ability (. The results showed that Fresta-F and Aromex-ME, two kinds of compound plant extracts, had total antioxidation capacity in serum for 44 days in the middle of the experiment. The level of malondialdehyde and the activity of superoxide dismutase (SOD) were significantly improved by P0.05A, but not by P0.05N in the late stage of the experiment. The two compound plant extracts Fresta-F and Aromex-ME had the ability of scavenging free radical and reducing ability to some extent, but both were lower than that of BHT. to sum up. The plant extracts Fresta-F and Aromex-ME could improve the performance of weaning piglets, reduce blood lipid and improve the antioxidation ability of early weaning piglets.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S828.5

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