一种新抗球虫中药组方及其与DVD联合用药对鸡球虫感染的疗效研究

发布时间：2018-01-19 01:20

本文关键词： 中药 DVD 鸡柔嫩艾美尔球虫抗球虫指数　出处：《河南科技大学》2017年硕士论文　论文类型：学位论文

【摘要】：鸡球虫病是养殖生产过程中造成严重损失的疾病之一。目前临床上治疗球虫仍以化学抗球虫药为主,其使用广泛,尤其是常作为饲料添加剂。由于球虫类型以及生殖阶段(有性生殖阶段、无性生殖阶段和孢子生殖阶段)的特殊性,造成了在球虫病的防治中要根据不同的阶段采用不同的药物,临床上根据病情发展经常选择两种或两种以上的药物联合用药才能达到较好的治疗效果。随着临床对化学药物的使用次数和剂量的增加,球虫的耐药性逐渐增强,而且化药的使用对动物机体造成的伤害很大,特别是磺胺类药物的大剂量使用不但有肾毒性而且还有免疫抑制作用。中草药无耐药性、无不良反应、无免疫抑制、无休药期、无毒副作用,而且对球虫病也有很好的预防和治疗效果,因此加强研究中草药对球虫病的防治意义更加深远。为此,本研究在传统中药组方鸡球虫散的基础上,为了增加杀虫效果,去掉何首乌,加入常山,构成新的抗球虫中药组方,并考察该组方与DVD联合使用对球虫的治疗效果,以解决中药起效慢、治疗时间长的问题,又能减少化学药物的使用,一定程度也能降低用药成本,进而更好的起到治疗球虫病的目的。本研究具体内容如下:1.筛选最佳抗鸡柔嫩艾美尔球虫中药组方比例试验为了筛选中药抗球虫组方(青蒿、常山、仙鹤草、白头翁、肉桂)中青蒿与常山的最佳比例,制备了3个拟定组方(青蒿与常山比例分别为1:0.5、1:1、1:2),通过3个组方对人工感染鸡柔嫩艾美尔球虫病,以20g/kg饲料的剂量,进行治疗试验,考察3个组方对人工感染鸡球虫病的治疗效果。其实验数据为:存活率分别为:83%、90%、87%;相对增重率分别为:86.77%、94.43%、92.12%;抗球虫指数分别为:163.77、179.83、174.72。结果果表明:3个组方对鸡艾美尔球虫病均有明显的治疗作用,其中组方2(青蒿与常山比例为1:1)的作用效果最好。故采用该组方进行后续研究。2.筛选新中药组方与DVD联合用药最佳剂量为筛选出抗球虫中药组方(青蒿、常山、仙鹤草、白头翁、肉桂)与抗菌增效剂DVD(40 ppm))联合使用对人工感染鸡柔嫩艾美尔球虫球虫病的治疗效果的最佳剂量,将3种不同浓度组方中药:分别为第1组:10 g/kg饲料的低剂量中药组、第2组:20g/kg饲料的中剂量中药组、第3组:30 g/kg饲料的高剂量中药组,与DVD(40PPM)联合使用、第4组:不用中药只用DVD(80 ppm)组、第5组空白对照组、第6组攻毒对照组,在饲料中混合均匀,感染球虫的同时给药。试验结果前4组的其存活率分别为:93%、97%、97%、83%;其盲肠病变值分别为:4.8、4.5、4.3、5.2;卵囊值分别为:1、0、0、1;抗球虫指数分别为:173.8、181.77、187.79、160.83,第2组中剂量和第3组高剂量中药组均能达到高效抗球虫作用。从经济效益考虑以第2组中剂量中药组(20 g/kg)饲料与DVD(40 ppm/d)联合应用做为新中药组方进行后续研究。3.新中药组方与DVD联合用药防治鸡球虫病的临床试验为确定抗球虫新中药组方与抗菌增效剂DVD联合使用对自然感染鸡柔嫩艾美尔球虫球虫病的治疗效果,按推荐剂量把两者联合使用,在饲料中混合均匀,药物对照组使用鸡球虫散进行治疗,给药后第5 d,供试药物组和药物对照组对鸡球虫卵囊减少率分别为99.12%和98.80%,鸡只存活率比药物对照组高0.13%,料肉比与药物对照组相比降低0.17。结果证明药物以20 g/kg饲料剂量与DVD(40ppm/d)联合拌料,连用5 d,对鸡球虫病的治疗效果显著高于传统的鸡球虫散。研究结果表明:青蒿与常山新型抗球虫组方的最佳比例(1:1)对鸡艾美尔球虫病具有良好的治疗作用,其对实验鸡的保护率达90%,ACI为179.83。青蒿与常山抗球虫组方(1:1)与DVD(40 ppm/d)联合用药的最佳剂量为20g/kg饲料,连续使用5 d,对人工感染和自然感染柔嫩艾美尔球虫病鸡有良好的治疗效果。
[Abstract]:Chicken coccidiosis is one of the serious loss of aquaculture production in the process of disease. The clinical treatment of coccidiosis is mainly chemical anticoccidial drugs, it is widely used, especially used as feed additives. Because of coccidia types and reproductive stages (stages of sexual reproduction, asexual stage and spore stage) particularity caused to according to the different stages of the different drugs in the prevention and treatment of coccidiosis, according to the clinical progression of the disease often choose two or more than two kinds of drugs in order to achieve a better therapeutic effect. With the increasing use of chemical drugs in the clinical dose and frequency of the coccidial drug resistance increases, and the use of drugs to cause the animal body hurts a lot, especially large doses of sulfonamides use not only has renal toxicity and immunosuppressive effect of Chinese herbal medicine. No drug resistance, no No adverse reaction, immune suppression, no withdrawal period, no toxic side effect, but also has a very good prevention and treatment of coccidiosis, so the significance of Chinese herbal medicine to strengthen the prevention and control of coccidiosis is more profound. Therefore, this study based on the traditional Chinese medicine prescription of Eimeria powder, in order to increase the insecticidal effect, remove Polygonum multiflorum join, Changshan, constitute a new anticoccidial prescription of traditional Chinese medicine, and the effects of the prescription and DVD combination on coccidiosis treatment effect, in order to solve the slow onset of traditional Chinese medicine, treatment time is long, but also can reduce the use of chemical drugs, to a certain extent can reduce the drug cost, and better play to the treatment of coccidiosis specific contents of this study are as follows: 1. the selection of the best anti chicken Eimeria tenella in order to test the proportion of prescription of traditional Chinese medicine screening of traditional Chinese medicine prescription anticoccidiosis (Artemisia annua, Changshan, agrimony, Pulsatilla, cinnamon) in Artemisia annua and Changshan The best proportion of preparation of 3 prescription (Artemisia and Changshan to respectively 1:0.5,1:1,1:2), through 3 prescriptions on artificial infection of Eimeria tenella in chickens, to feed 20g/kg dose, treatment test, to investigate the therapeutic effect of 3 prescriptions on artificial infected chicken coccidiosis. The experimental data: the survival rate: 83%, 90%, 87%; the relative growth rates were: 86.77%, 94.43%, 92.12%; anticoccidiosis index were: 163.77179.83174.72. result showed that 3 components on the obvious curative effect has the chicken Eimeria coccidiosis, prescription 2 (Artemisia annua and Changshan 1:1 ratio) the best effect. So the use of the prescription the follow-up study of.2. screening for screening new anticoccidial prescription of traditional Chinese medicine prescription of traditional Chinese medicine combined with DVD dosage (Artemisia annua, Changshan, agrimony, Pulsatilla, cinnamon) and antibacterial synergist DVD (40 ppm) combined with the use of artificial sense) The optimal dose of chicken Eimeria tenella coccidiosis infection treatment effect, the concentration of 3 different herbs respectively into first groups: low dose group, 10 g/kg feed second groups: middle dose traditional Chinese medicine group, 20g/kg feed third groups: high dose group 30 g/kg feed, DVD (40PPM) combined with use of fourth groups: do not use the traditional Chinese medicine with DVD (80 ppm) group, fifth groups of blank control group, sixth groups of controlgroup, uniformly mixed in the feed, while administration of coccidian infection. The test results of 4 groups before the survival rate were 93%, 97%, 97%, 83%; the cecal lesions values were: 4.8,4.5,4.3,5.2 = 1,0,0,1; oocysts; anticoccidiosis index were: 173.8181.77187.79160.83, third groups of high dose and dose of Chinese medicine group in the second groups were able to achieve efficient anticoccidial effect. From the economic consideration to the pharmaceutical dose in the second group (20 g/kg) in feed with DVD (40 ppm/d) combined with The application for clinical trials of new prescription for subsequent research on.3. new prescription of traditional Chinese medicine combined with DVD coccidiosis in chickens to determine the new anticoccidial prescription of traditional Chinese medicine combined with natural infection of Eimeria tenella coccidiosis treatment effect of antibacterial synergist DVD, according to the recommended dose to the combination, uniformly mixed in the feed, drugs the control group using chicken coccidia powder treatment, fifth d after administration, the drug group and drug control group reduced rates were 99.12% and 98.80% of chicken coccidian oocysts in chickens, the survival rate is 0.13% higher than the drug control group, the ratio of feed and the drug control group decreased compared with 0.17. results in a 20 g/kg dose of feed with DVD (40ppm/d) combined with mixing, for 5 d, the chicken coccidiosis treatment effect was significantly higher than that of the traditional chicken coccidia powder. The results showed that the optimal ratio of Artemisia annua and Changshan new anticoccidial prescription Cases (1:1) has good therapeutic effect on chicken Eimeria coccidiosis, the experimental chicken protection rate of 90%, ACI 179.83. and Changshan Artemisia anticoccidiosis group (1:1) and DVD (40 ppm/d) the best dosage combination for 20g/kg feed, continuous use of 5 d treatment has a good effect on Eimeria chicken coccidiosis artificial infection and natural infection.

【学位授予单位】：河南科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.31

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