部分鸡种Brd2基因SNP和INDEL分析

发布时间：2018-01-20 08:49

本文关键词： 鸡 Brd基因 SNP Indel 进化树　出处：《安徽农业大学学报》2017年02期 　论文类型：期刊论文

【摘要】：Brd2(bromodomain containing protein 2)基因位于MHC(major histocompatibility complex)基因家族中,探究不同鸡种Brd2基因的SNP和Indel位点具有重要意义。试验选取我国26个优良的地方鸡种,对Brd2基因进行目标捕获测序,以NCBI中原鸡(Gallus gallus)的Brd2基因序列为参照,筛选出Brd2基因外显子和内含子中的SNP和Indel以及氨基酸的改变位点,最后制作进化树。在26个鸡种中,Brd2基因的外显子筛选出25个SNP位点,没有检测到Indel位点;内含子筛选出30个Indel位点,没有筛选出SNP位点;外显子中氨基酸仅有4个发生错义突变,其余均发生同义突变。从进化树看出,26个鸡种分为3大类:安卡鸡、雪山草鸡、文昌鸡、北京油鸡和萧山鸡类聚在一起;泰和乌骨鸡、SPF-来航鸡、河南斗鸡和广西黄鸡类聚在一起;剩余的鸡种类聚在一起,但同源性相对较低。在我国优质鸡种中Brd2基因具有一定的保守性,不同鸡种同源性较低。
[Abstract]:Brd2(bromodomain containing protein 2 gene is located in MHC2. In the major histocompatibility complex family. It is of great significance to explore the SNP and Indel loci of Brd2 gene in different chicken breeds. 26 excellent local chicken breeds in China were selected and the Brd2 gene was sequenced by target capture. The Brd2 gene sequence of Gallus gallus in NCBI was used as reference. The mutation sites of SNP and Indel and amino acids in exon and intron of Brd2 gene were screened. Finally, the evolutionary tree was made in 26 chicken breeds. 25 SNP loci were screened out of the exon of Brd2 gene, and no Indel loci were detected. 30 Indel loci were screened in intron, but no SNP loci were screened. Only 4 amino acids had missense mutations in exons, while the others had synonymous mutations. From the evolutionary tree, 26 chicken breeds were classified into 3 groups: Anka chicken, snow mountain grass chicken and Wenchang chicken. Beijing oil chicken and Xiaoshan chicken together; Taihe Black-bone Chicken (SPF-Laihang), Henan Cockfight and Guangxi Yellow Chicken were gathered together. The remaining chicken species were clustered together, but the homology was relatively low. The Brd2 gene was conserved to some extent and the homology was lower in different breeds in China.
【作者单位】：扬州大学动物科学与技术学院;
【基金】：国家自然科学基金项目(31301966) 江苏省六大人才高峰计划共同资助
【分类号】：S831.2
【正文快照】： BET(bromodomain and extra C-terminaldomain)家族蛋白是新发现的一类转录调节因子(transcriptional regulator),其典型特征是具有两个串联的Bromodomain,并且在C端有一个ET域[1]。Brd2基因又称Ring3,含有2个溴功能结构域(bromodomain,BRD),属于BET蛋白家族中的一员[2]。鸡的

【相似文献】