麦麸阿魏酰低聚糖对敌草快致氧化应激大鼠血浆和组织抗氧化能力的影响

发布时间：2018-01-20 23:45

本文关键词： 麦麸阿魏酰低聚糖断奶大鼠氧化应激抗氧化能力　出处：《动物营养学报》2017年12期 　论文类型：期刊论文

【摘要】：本试验以酿酒酵母和枯草芽孢杆菌作为混合菌种发酵小麦麸皮,通过Amberlite XAD-2柱分离纯化制备麦麸阿魏酰低聚糖(feruloyl oligosaccharides,FOs),探讨麦麸FOs对敌草快(diquat)诱导的大鼠氧化应激是否有缓解作用。试验选用体重相近的断奶雄性大鼠48只,随机分为未攻毒组、攻毒组、攻毒+100 mg/kg BW麦麸FOs组、攻毒+200 mg/kg BW麦麸FOs组、攻毒+300 mg/kg BW麦麸FOs组和攻毒+100 mg/kg BW维生素C组,每组8个重复,每个重复1只鼠,各组大鼠均饲喂相同的商业饲料。麦麸FOs和维生素C配制成水溶液,采用灌胃的方式给予,未攻毒组、攻毒组用生理盐水替代,灌胃体积0.2 mL,连续灌胃15 d。灌胃结束当天,未攻毒组大鼠注射0.3 mL生理盐水,其他5组按0.1 mmol/kg BW的剂量腹腔注射0.3 mL敌草快。敌草快攻毒12 h后取样,分析各组大鼠血浆以及肝脏、肾脏和回肠中总抗氧化能力(T-AOC),过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性以及谷胱甘肽(GSH)和8-羟基脱氧鸟苷(8-OHd G)的含量。结果显示:1)通过混菌发酵小麦麸皮制备麦麸FOs,利用Amberlite XAD-2柱进行分离纯化,获得的麦麸FOs浓度为0.059 mmol/g。2)腹腔注射敌草快显著降低大鼠血浆中SOD活性和GSH含量(P0.05),显著降低大鼠肝脏中T-AOC,CAT、GSH-Px活性及GSH含量(P0.05),显著降低大鼠肾脏中T-AOC及CAT、SOD活性(P0.05),显著降低大鼠回肠中T-AOC,CAT、GSH-Px活性及GSH含量(P0.05),并显著提高大鼠血浆和各组织中8-OHd G含量(P0.05)。3)在敌草快引起的氧化应激状态下,灌胃一定剂量的麦麸FOs可以显著提高大鼠血浆中SOD(400 mg/kg BW)、GSH-Px活性(100和200 mg/kg BW)以及GSH含量(100和200 mg/kg BW)(P0.05),显著提高大鼠肝脏中T-AOC(100、200和400 mg/kg BW),CAT(200和400 mg/kg BW)、SOD(100、200和400 mg/kg BW)和GSH-Px活性(100、200和400 mg/kg BW)以及GSH含量(100、200和400 mg/kg BW)(P0.05),显著提高大鼠肾脏中T-AOC(400 mg/kg BW),CAT(200 mg/kg BW)和GSH-Px活性(200和400 mg/kg BW)以及GSH含量(400 mg/kg BW)(P0.05),显著提高大鼠回肠中T-AOC(200 mg/kg BW),SOD(400 mg/kg BW)和GSH-Px活性(100、200和400 mg/kg BW)以及GSH含量(100、200和400 mg/kg BW)(P0.05),显著降低血浆和各组织中8-OHd G含量(血浆、肾脏、回肠:100、200和400 mg/kg BW;肝脏:100 mg/kg BW)(P0.05);且灌胃200、400 mg/kg BW麦麸FOs后,大鼠血浆和组织中部分抗氧化相关指标可恢复到正常生理状态水平。综上所述,本试验制备的麦麸FOs可以通过有效提高大鼠血浆和组织中抗氧酶活性和GSH含量,降低DNA氧化应激代谢产物8-OHd G的含量,有效缓解由敌草快诱导产生的氧化应激。
[Abstract]:The fermentation of wheat bran by Saccharomyces cerevisiae and Bacillus subtilis was studied. Wheat bran feruloyl oligosaccharidesof wheat bran was purified by Amberlite XAD-2 column. To investigate whether wheat bran FOs can relieve oxidative stress induced by diquat), 48 weaned male rats with similar body weight were randomly divided into control group and control group. 100 mg/kg BW wheat bran FOs group, 200 mg/kg BW wheat bran FOs group. #number0# mg/kg BW wheat bran FOs group and 100 mg/kg BW BW vitamin C group were treated with 8 replicates in each group and 1 rat in each group. The rats in each group were fed the same commercial feed. The wheat bran FOs and vitamin C were prepared into aqueous solution. The rats in the control group were given by gavage. The rats in the control group were replaced by normal saline and the volume was 0.2 mL. The rats in the control group were injected 0.3 mL of normal saline on the day of the end of oral administration. The other five groups were injected intraperitoneally with 0. 3 mmol/kg BW. The plasma and liver of each group were analyzed. Total antioxidant capacity in kidney and ileum is T-AOC, catalase (CAT). Activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and glutathione (GSH) and 8-hydroxydeoxyguanosine (8-OHd GG). The results showed that wheat bran FOs was prepared by mixed fermentation of wheat bran. Amberlite XAD-2 column was used for separation and purification. The concentration of FOs in wheat bran was 0.059 mmol / g. 2) the plasma SOD activity and GSH content of rats were significantly decreased by intraperitoneal injection of diphacinone (P0.05). The activity of GSH-Px and the content of GSH in rat liver were significantly decreased, and the T-AOC and CAT in kidney were significantly decreased. The activity of SOD (P0.05) significantly decreased the activity of GSH-Px and the content of GSH (P0.05) in rat ileum. The content of 8-OHdG in plasma and tissues of rats was significantly increased under the oxidative stress induced by dimethylol. A certain dose of wheat bran FOs could significantly increase the level of SOD(400 mg/kg BW in plasma of rats. The activity of GSH-Px was 100 and 200 mg/kg BW) and the content of GSH was 100 and 200 mg/kg respectively (P0.05). The contents of T-AOC in the liver of rats were significantly higher than that in the control group (P < 0.05). The contents of T-AOC in the liver of rats were significantly higher than that in the control group (P < 0.05). 200 and 400 mg/kg BW) and GSH-Px activity 100 mg/kg BW200 and 400 mg/kg BW) and GSH content 100. 200 and 400 mg/kg BWN (P0.05) significantly increased the T-AOC (400 mg/kg BW) in the kidney of rats. CAT(200 mg/kg BW and GSH-Px activity 200 and 400 mg/kg BW) and GSH content of 400 mg/kg BW (. P0.05). The activity of T-AOC (200 mg/kg) and GSH-Px (400 mg/kg BW) in ileum of rats were significantly increased. The contents of 200 and 400 mg/kg BWs and the contents of GSH were 100 mg/kg and 400 mg/kg respectively (P0.05). The levels of 8-OHdG in plasma, kidney, ileum and ileum were significantly decreased (plasma, kidney, ileum, 200 and 400 mg/kg BWs). Liver: 1. 100 mg/kg BWN. P0. 05; After 200,400 mg/kg BW wheat bran FOs, some antioxidant related indexes in plasma and tissue of rats could return to normal physiological state. The wheat bran FOs prepared in this experiment can effectively increase the activity of antioxidant enzyme and the content of GSH in plasma and tissue of rats, and decrease the content of 8-OHdG, a metabolite of oxidative stress of DNA. It can effectively alleviate oxidative stress induced by diphthoxylum quinquefolium.
【作者单位】：内蒙古农业大学动物科学学院;
【基金】：内蒙古农业大学引进人才科研启动项目(YJ2015-1) 内蒙古自治区科技重大项目
【分类号】：S859.7
【正文快照】： 阿魏酸(ferulic acid,FA)是桂皮酸的衍生物之一,是禾本科植物中普遍存在的一种酚酸,主要与细胞壁多糖和木质素交联构成细胞壁的一部分[1]。阿魏酰低聚糖(feruloyl oligosaccharides,FOs)是FA的羧基与低聚糖的羟基通过酯键连接而成的一类重要化合物。由于通过酯键连接的低聚糖

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