高精料日粮对奶山羊乳腺组织NOD1炎性信号通路的影响

发布时间：2018-01-21 08:07

本文关键词： 奶山羊高精料日粮丁酸钠乳腺炎症反应　出处：《畜牧与兽医》2017年09期 　论文类型：期刊论文

【摘要】：本试验旨在研究高精料日粮对奶山羊乳腺组织NOD1炎性信号通路的影响与调控。选取18头泌乳中期的健康萨能奶山羊,试验前分别安装瘤胃瘘管和乳静脉血管插管,随机分成3组:饲喂精粗比为4∶6的低精料日粮作为对照组(LC);饲喂精粗比为6∶4的高精料日粮作为处理组(HC);饲喂高精料日粮并添加丁酸钠作为调控组(BHC),试验时间为20周。试验结束后采集瘤胃液、乳静脉血和乳腺组织样品用于后续的瘤胃pH值、乳静脉血中炎性因子浓度、乳腺组织中炎症相关基因m RNA和蛋白水平的检测。结果显示:一天内HC组的瘤胃pH值小于5.8持续4 h且显著低于LC组,表明亚急性瘤胃酸中毒(SARA)诱导成功;饲喂4 h后BHC组瘤胃pH值显著高于HC组而与LC组差异不显著,表明丁酸钠能够缓解瘤胃pH的持续降低。HC组乳静脉血中炎性细胞因子IL-1β、IL-6和TNF-α的浓度显著高于LC组,而BHC组的炎性细胞因子溶度显著低于HC组并与LC组无显著差异;与LC组和BHC组相比,HC组奶山羊乳腺组织炎症相关基因NOD1、RIP2、NF-κB、IL-1β、IL-6的表达量显著上调;NOD1、NF-κBp65、NF-κBpp65的蛋白表达水平和m RNA水平变化一致。结果表明,长期饲喂高精料日粮能够激活奶山羊乳腺组织的NOD1-NF-κB炎性信号通路,诱导乳腺炎症应答,日粮中添加丁酸钠可以一定程度地缓解乳腺组织的炎症反应。
[Abstract]:The aim of this study was to study the effect and regulation of high concentrate diet on NOD1 inflammatory signaling pathway in breast tissue of dairy goats. Eighteen healthy Sanen dairy goats with middle lactation were selected. Before the trial, rumen fistula and mammary vein catheterization were installed, and were randomly divided into three groups: the control group was fed with a low concentrate diet of 4: 6 as the control group; The high concentrate ration of 6: 4 was used as the treatment group. High concentrate diet was fed with sodium butyrate as control group for 20 weeks. Rumen fluid, breast blood and breast tissue samples were collected for subsequent rumen pH value at the end of the experiment. The concentration of inflammatory factors in mammary vein blood. The results showed that the rumen pH value of HC group was less than 5.8 for 4 h and significantly lower than that of LC group. The results showed that subacute rumen acidosis was induced successfully. The rumen pH value of BHC group was significantly higher than that of HC group after 4 h feeding, but there was no significant difference between LC group and BHC group. The results showed that sodium butyrate could alleviate the continuous decrease of rumen pH. The concentrations of IL-1 尾 -IL-6 and TNF- 伪 in mammary vein blood of HC group were significantly higher than those of LC group. The solubility of inflammatory cytokines in BHC group was significantly lower than that in HC group and there was no significant difference between LC group and BHC group. Compared with LC group and BHC group, the expression of Nord1, RIP2, NF- 魏 B, IL-1 尾 and IL-6 in mammary tissue of dairy goats in HC group was significantly upregulated. The protein expression level of NF- 魏 B p65 Bpp65 was consistent with that of m RNA. The results showed that the protein expression level of NF- 魏 B p65 was the same as that of m RNA. Feeding high concentrate diet for a long time can activate the NOD1-NF- 魏 B inflammatory signaling pathway in breast tissue of dairy goats and induce the mammary inflammation response. Sodium butyrate in diet can alleviate the inflammation of breast tissue to some extent.
【作者单位】：南京农业大学动物医学院;
【基金】：国家自然科学基金项目(31672618)
【分类号】：S827.5
【正文快照】： 随着对乳制品需求量的增加,反刍动物养殖业中普遍通过增加日粮中的精料比例来提高产奶性能,但过多的精料在瘤胃内迅速发酵容易引起挥发性脂肪酸(VFA)和乳酸的蓄积,引发亚急性瘤胃酸中毒(SARA)[1]。碳水化合物在瘤胃微生物的作用下迅速发酵,短时间内生成大量的挥发性脂肪酸,超

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