秦川牛PSMC2基因的SNP及其对体尺和胴体性状的影响

发布时间：2018-01-21 12:34

本文关键词： 秦川牛 PSMC2 多态性体尺胴体性状　出处：《西北农林科技大学》2015年硕士论文　论文类型：学位论文

【摘要】：体尺和胴体性状是秦川牛肉牛重要的经济性状,是受到多个基因控制的数量性状。本研究旨在分析秦川牛PSMC2基因外显子的多态性及其与秦川牛体尺和胴体性状的关联性。本实验选择171头相同饲养条件下24-30月龄的健康秦川牛去势公牛,采用DNA测序技术,检测PSMC2基因的SNP位点,结果表明:PSMC2基因在扩增序列的第131位、基因组的第4495565位处发生一处单碱基CG的突变;在序列第198位、基因组第4495632位发生一处AG突变;在序列第265位、基因组第4495699位发生一处CG突变以及在序列第285位、基因组第4495719位发生一处CG突变。运用SPSS统计软件中的GLM模型,将发现的SNP位点与体尺及胴体性状进行关联性分析,发现C131G基因座与空腹24 h宰前活重指标显著相关,其中CG基因型个体显著高于CC基因型(P0.05),在整个群体中出现了CG基因型优于CC基因型的趋势;A198G基因座在6个体尺及胴体指标上两种基因型个体差异均不显著(P0.05),但在群体中出现了AG基因型优于AA基因型的趋势;C265G基因座中三种基因型个体在三个体尺指标及屠宰率指标上无显著差异(P0.05),在宰前活重、胴体重指标上出现了CCGGCG的趋势,且CC基因型显著高于CG基因型(P0.05)。C285G基因座中三种基因型个体与腰角宽、胴体重指标显著相关,在腰角宽指标上GG基因型显著高于CG基因型(P0.05),在宰前活重指标上CC基因型显著高于GG基因型(P0.05),在胴体重指标上CC基因型极显著高于GG基因型个体(P0.01),并且群体中宰前活重和胴体重指标出现了CCCGGG的趋势。结果提示,秦川牛PSMC2基因中存在SNP位点且与部分体尺和胴体性状显著相关,可考虑作为标记辅助选择中的分子标记,为秦川肉牛留种、选育提供参考,从而加快具有优质经济性状牛种群的建立。
[Abstract]:Body size and carcass traits are important economic traits of Qinchuan beef cattle. The aim of this study was to analyze the polymorphism of PSMC2 gene exon of Qinchuan cattle and its association with body size and carcass traits of Qinchuan cattle. Under the condition of 24-30 months old healthy Qinchuan cattle ovariectomized bull. DNA sequencing technique was used to detect the SNP site of PSMC2 gene. The results showed that the PSMC2 MC2 gene was in the 131st position of the amplified sequence. A single base CG mutation occurred at the 4495565 position of the genome. A AG mutation occurred at the 198th position of the sequence and at the 4495632 position of the genome. A CG mutation occurred at the 4495699 position of the genome and at the 285th position of the sequence. A CG mutation occurred at the 4495719 position of the genome. Using the GLM model in the SPSS statistical software, the relationship between the SNP loci and body size and carcass traits was analyzed. It was found that the C131G locus was significantly correlated with the fasting 24 h pre-mortem weight, and the CG genotype was significantly higher than the CC genotype (P0.05). CG genotype was superior to CC genotype in the whole population. There was no significant difference between the two genotypes in the size of 6 individuals and carcass index in A198G locus, but there was a trend that AG genotype was superior to AA genotype in population. There was no significant difference among the three genotypes in the three genotypes of C265G locus in terms of body size and slaughter rate, but there was a trend of CCGGCG in the premortem weight and carcass weight index. CC genotype was significantly higher than that of CG genotype (P0.05G. C285G locus). The three genotypes were significantly correlated with waist angle width and carcass weight index. GG genotype was significantly higher than CG genotype P0.05L in waist angle width index, and CC genotype was significantly higher than GG genotype P0.05 in pre-mortem weight index. In carcass weight index, CC genotype was significantly higher than GG genotype individuals (P0.01), and there was a trend of CCCGGG in pre-slaughter weight and carcass weight index. There is a SNP locus in the PSMC2 gene of Qinchuan cattle, which is significantly related to some body size and carcass traits. It can be considered as a molecular marker in marker-assisted selection to provide reference for breeding and breeding of Qinchuan beef cattle. So as to accelerate the establishment of cattle population with high quality economic traits.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823

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