猴头菇多糖对MDRV感染番鸭血清中细胞因子及激素水平的影响

发布时间：2018-01-21 22:24

本文关键词： 番鸭呼肠孤病毒猴头菇多糖细胞因子激素　出处：《中国兽医科学》2017年02期 　论文类型：期刊论文

【摘要】：为研究猴头菇多糖(HPS)对番鸭呼肠孤病毒(MDRV)感染番鸭血清中的细胞因子及激素的影响,以探讨HPS对MDRV感染雏番鸭所致免疫抑制免疫调节作用。本试验借助已建立的MDRV自然感染模型进行HPS防治MDRV感染的试验,采用放射免疫测定方法检测试验番鸭血清中部分细胞因子和激素含量的动态变化。结果显示,MDRV感染能抑制番鸭机体分泌IL-1、IL-2、IL-4、生长激素(GH)、促肾上腺皮质激素(ACTH),在感染中后期促进试验番鸭机体分泌糖皮质激素(GC),引起免疫应答下降或停止;HPS预防用药可以促进MDRV感染番鸭分泌IL-1、IL-2和IL-4;维持ACTH含量的相对稳定,抑制MDRV感染番鸭GC含量的急剧升高,促进GH的分泌,调节神经-内分泌-免疫系统。研究表明,HPS预防用药可通过调节MDRV感染番鸭部分细胞因子和激素的分泌量,参与机体免疫调节的过程,缓解MDRV感染番鸭的临床症状及病理变化,降低死亡率,促进免疫抑制番鸭的康复,为HPS在临床上防治MDRV的研究提供理论依据。
[Abstract]:To study the effect of HPSs on cytokines and hormones in serum of muscovy duck infected with MDRV. The aim of this study was to investigate the immunosuppressive immunomodulation effect of HPS on MDRV infected young muscovy ducks. In this study, HPS was used to prevent and cure MDRV infection with the help of the established MDRV natural infection model. The dynamic changes of cytokines and hormones in serum of muscovy ducks were detected by radioimmunoassay. The results showed that MDRV infection could inhibit the secretion of IL-1and IL-2 in muscovy ducks. IL-4, GH, ACTH, promoted the secretion of glucocorticoid in muscovy duck at the middle and late stage of infection, and caused the decrease or stop of immune response. HPS prophylaxis can promote the secretion of IL-1IL-2 and IL-4 in muscovy ducks infected with MDRV. Maintain the relative stability of ACTH content, inhibit the rapid increase of GC content in muscovy ducks infected with MDRV, promote the secretion of GH, regulate the neuro-endocrine-immune system. HPS prophylaxis can regulate the secretion of cytokines and hormones in Muscovy ducks infected with MDRV, participate in the process of immune regulation, and alleviate the clinical symptoms and pathological changes of Muscovy ducks infected with MDRV. Reducing mortality and promoting the recovery of immunosuppressive muscovy ducks provide theoretical basis for the clinical study of HPS in the prevention and treatment of MDRV.
【作者单位】：福建农林大学动物科学学院;福建省兽医中药与动物保健重点实验室;
【基金】：国家自然科学基金项目(31372474) 国家自然科学基金—促进海峡两岸科技合作联合基金项目(U1305212)
【分类号】：S858.32
【正文快照】： 番鸭呼肠孤病毒(muscovy duck reovirus,MDRV)感染最早由Kaschula等[1]在南非报道,其主要侵害4~45日龄的番鸭,死亡率很高,临床上以软脚、腹泻等为主要症状,以肝、脾肿大及灰白色坏死点等为主要病变,耐过番鸭常出现明显的生长停滞。MDRV在感染宿主多个组织器官的同时,可以诱导

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