猪繁殖与呼吸综合征病毒N蛋白与宿主细胞DDX5蛋白相互作用研究

发布时间：2018-01-21 23:55

本文关键词： 猪繁殖与呼吸综合征病毒 N蛋白 DDX 相互作用　出处：《中国动物传染病学报》2017年03期 　论文类型：期刊论文

【摘要】：研究利用免疫共沉淀的方法证明猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)N蛋白与MARC-145细胞DDX5蛋白之间存在相互作用,且两种蛋白共定位于MARC-145细胞质内。利用构建的EGFP-N和m Cherry-DDX5真核表达载体共转染MARC-145细胞后,发现N蛋白并不能将DDX5蛋白募集至细胞质。采用si RNA沉默MARC-145细胞DDX5基因表达之后,病毒复制水平上升;荧光定量检测结果显示,DDX5沉默表达也能够明显促进病毒基因组RNA和长链亚基因组RNA的转录水平。结果表明DDX5参与了病毒复制转录过程并对病毒增殖具有抑制作用。
[Abstract]:To study the evidence of porcine reproductive and respiratory syndrome virus (PRRSV) by immunoprecipitation. Porcine reproductive and respiratory syndrome virus. There is interaction between PRRSV)N protein and DDX5 protein in MARC-145 cells. The two proteins were co-localized in the cytoplasm of MARC-145. The constructed EGFP-N and m Cherry-DDX5 eukaryotic expression vectors were co-transfected into MARC-145 cells. It was found that N protein could not raise DDX5 protein to cytoplasm. After si RNA was used to silence the expression of DDX5 gene in MARC-145 cells, the replication level of the virus increased. Fluorescence quantitative analysis showed that. The silencing expression of DDX5 also significantly promoted the transcription level of viral genomic RNA and long chain subgenomic RNA. The results showed that DDX5 was involved in viral replication and transcription and inhibited virus proliferation. .
【作者单位】：中国农业科学院上海兽医研究所;
【基金】：国家科技支撑项目(2015BAD12B01-1) 国家自然科学基金项目(31502072,31302098,31300140)
【分类号】：S852.651
【正文快照】： CHARACTERIZATION OF INTERACTION BETWEEN NUCLEOCAPSIDPROTEIN OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROMEVIRUS AND HOST DDX5 PROTEINLIU Huan,HUANG Qin-feng,ZHANG Wen-chao,JIANG Yi-feng,YANG Shen,GAO Fei,ZHOU Yan-jun,TONG Guang-zhi(Shanghai Veterinary

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