N-羟甲基蛋氨酸钙水平对泌乳期奶牛血清含氮物和游离氨基酸的影响

发布时间：2018-01-23 05:33

本文关键词： 泌乳期奶牛 N-羟甲基蛋氨酸钙含氮物游离氨基酸　出处：《中国畜牧兽医》2017年03期 　论文类型：期刊论文

【摘要】：为研究N-羟甲基蛋氨酸钙(N-hydroxymethyl methionine calcium,N-HMM-Ca)对荷斯坦奶牛血清含氮物和游离氨基酸的影响,试验选用胎次、产奶量和泌乳天数相近的30头健康荷斯坦奶牛,随机分为5组。对照组饲喂基础日粮,试验组分别在基础日粮中添加0.15%、0.30%、0.75%和1.50%N-HMM-Ca,预试期14d,正试期42d。结果显示:1日粮中补充N-HMM-Ca对奶牛血清中总蛋白、白蛋白、球蛋白含量无显著影响(P0.05)。第14天,1.5%组血清肌酐浓度显著低于对照组(P0.05);第42天,1.5%组尿素氮浓度显著低于对照组(P0.05),而0.75%组尿酸浓度极显著高于其他组(P0.01)。2第14天,0.15%组血清中谷氨酸、丝氨酸、精氨酸、亮氨酸、异亮氨酸、赖氨酸及总游离氨基酸浓度最高,与对照组相比差异显著或极显著(P0.05;P0.01);试验第42天,0.15%组血清中精氨酸、胱氨酸及总游离氨基酸浓度最高。0.30%组血清中谷氨酸、丝氨酸、酪氨酸、蛋氨酸、缬氨酸、赖氨酸浓度最高,与对照组相比差异显著或极显著(P0.05;P0.01)。综上所述,在基础日粮中添加0.15%、0.30%N-HMM-Ca对泌乳期奶牛血清含氮物含量无显著影响(P0.05),且添加0.15%N-HMMCa能够显著提高泌乳期奶牛血清游离氨基酸含量,综合考虑,N-HMM-Ca的添加量以0.15%为宜。
[Abstract]:To study N-hydroxymethyl methionine calcium of N-hydroxymethionine. The effects of N-HMM-Caon on serum nitrogen and free amino acids in Holstein cows were studied. Thirty healthy Holstein cows with similar milk yield and lactation days were selected. The control group was fed with basal diet, the experimental group was fed with 0.15% 0.30% and 1.50 N-HMM-Ca0.75% and 1.50 N-HMM-Ca1, respectively, for 14 days. The results showed that N-HMM-Ca supplementation had no significant effect on the contents of total protein, albumin and globulin in serum of dairy cattle on the 14th day. The serum creatinine concentration in the 1.5% group was significantly lower than that in the control group (P 0.05). The concentration of urea nitrogen in 1.5% group was significantly lower than that in the control group on the 42nd day, while the uric acid concentration in group 0.75% was significantly higher than that in other groups on the 14th day. The concentrations of glutamic acid, serine, arginine, leucine, isoleucine, lysine and total free amino acid were the highest in 0.15% group. P0.01; The concentration of arginine, cystine and total free amino acid in serum of 0.15% group was the highest on the 42nd day of the experiment. The concentration of glutamate, serine, tyrosine, methionine and valine in serum of 0.30% group was the highest. The concentration of lysine was the highest, and the difference was significant or extremely significant compared with the control group (P 0.05). To sum up, there was no significant effect of N-HMM-Ca on serum nitrogen content in lactating dairy cows (P0.05). The addition of 0.15% N-HMMCa could significantly increase the content of serum free amino acids in lactating dairy cows. The appropriate addition amount of N-HMM-Ca was 0.15%.
【作者单位】：扬州大学动物科学与技术学院;辽宁省畜产品安全监察所;
【基金】：江苏省高校优势学科建设工程项目(PAPD) 农业部农产品质量安全监管项目(2014) 长三角地区生鲜乳质量安全追溯体系关键技术研究应用项目(14395810100)
【分类号】：S823.5
【正文快照】： 蛋氨酸是泌乳奶牛饲喂高饲草日粮时的第一限制性氨基酸[1]。对高产奶牛来说,降解蛋白质和饲料蛋白质往往不能满足其生产需求,需要添加额外的过瘤胃蛋白。而动物蛋白质的营养实质上是氨基酸的营养,且过瘤胃蛋白的增加易引起瘤胃菌体蛋白质合成减少。因此,生产中常使用瘤胃保护

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