湖南健康猪源与流行性腹泻病猪肠球菌毒力基因检测分析

发布时间：2018-01-24 13:16

本文关键词： 肠球菌粪肠球菌屎肠球菌毒力基因溶血试验　出处：《中国预防兽医学报》2017年03期 　论文类型：期刊论文

【摘要】：为研究猪流行性腹泻(PED)病猪群中肠球菌及其携带毒力基因的变化情况,本研究采用PCR方法对115株猪源肠球菌检测其11种毒力基因的分布情况及进行了其溶血试验和明胶液化试验。结果,共检测到9种毒力基因:EF3314、efaA、gelE、ace、asal、cylA、esp、Hyl和EF0591,其中asa373和AS未检测到。72株健康猪源分离菌中毒力基因检出率分别为12.5%、9.7%、5.6%、2.8%、13.9%、4.2%、8.3%、15.3%和1.4%。43株PED病猪分离菌毒力基因阳性率分别为20.9%、25.6%、2.3%、18.6%、51.2%、11.6%、20.9%、0和7.0%。溶血试验结果显示115株肠球菌中溶血的占88.7%,其中α-溶血占40%,β-溶血占48.7%,共有8株检测到溶血素A基因(clyA),检出率为6.9%。11株粪肠球菌全部表现溶血,其中健康猪源分离株为α-溶血,PEDV感染猪分离株为β-溶血。明胶液化试验表明115株肠球菌中只有两株能液化明胶,均为粪肠球菌,一株为健康猪源分离株,一株为PEDV感染猪分离株,而且从这两株菌中均检测到了明胶酶E(gelE)基因。表明PED猪源肠球菌毒力基因检出率明显高于健康猪源肠球菌(p0.05)。明胶液化试验和溶血试验结果表明,粪肠球菌的这两种表型与基因型为肠球菌种中表达一致性是最高的,其携带毒力基因为肠球菌中所占比例最高。通过两种不同来源肠球菌毒力基因的比较,为PED的分析治疗与防控提供依据。
[Abstract]:For the study of porcine epidemic diarrhea (PED) in swine disease of Enterococcus and its virulence gene, this study uses the PCR method of 115 strains of Enterococcus from swine to determine the distribution of 11 kinds of virulence genes and the hemolytic test and gelatin liquefaction test was carried out. The results showed that 9 kinds of virulence genes were detected by EF3314. EfaA, gelE, ACE, asal, cylA, esp, Hyl and EF0591, asa373 and AS were not detected in isolates from healthy pigs virulence genes of.72 strains were detected in 12.5%, 9.7%, 5.6%, 2.8%, 13.9%, 4.2%, 8.3%, the positive rate of bacterial virulence genes PED and 1.4%.43 strains isolated from 15.3% pig disease were 20.9%, 25.6%, 2.3%, 18.6%, 51.2%, 11.6%, 20.9%, 0 and 7.0%. hemolysis test results showed that 115 strains of enterococci hemolysis accounted for 88.7% of the alpha beta hemolytic hemolysis accounted for 40%, accounted for 48.7%, a total of 8 strains were detected to hemolysin A gene (clyA), the detection rate of 6.9%.11 E. all soluble Enterococcus Blood of healthy pigs strain alpha hemolytic, PEDV swine isolates for beta hemolytic. Gelatin liquefaction test showed that only 115 strains of Enterococcus in two strains of liquefied gelatin, were Enterococcus faecalis, a strain of healthy swine isolates, a strain of swine infected with PEDV isolates, and from the two strains of bacteria were detected in the gelatinase E (gelE) showed that the porcine PED gene. Enterococcus virulence gene detection rate was significantly higher than that of healthy pigs Enterococcus (P0.05). Gelatin liquefaction test and hemolysis test results show that these two kinds of Enterococcus faecalis phenotypes and genotypes for enterococcal species in the form of consistency is the highest and the virulence gene in enterococci accounted for the highest proportion. By comparing the two different sources of virulence genes, provide the basis for the analysis of treatment and prevention and control of PED.

【作者单位】：湖南农业大学动物医学院;湖南畜禽安全生产协同创新中心;
【基金】：湖南省科技计划项目(2015 NK 3017)
【分类号】：S858.28
【正文快照】： 肠球菌是人和动物肠道内的一种正常菌群,也是一种人畜共患的机会性条件致病菌。常定植于肠道内,有较强的耐受性,当机体免疫力下降时,易导致菌群失调,肠球菌则大量繁殖,引起感染性疾病。目前文献报道的由肠球菌引起的感染性疾病越来越多,如脑膜炎、尿路感染、败血症、腹腔感染

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