FGF2和FGFR在牦牛繁殖周期子宫的表达与定位

发布时间：2018-01-25 09:16

本文关键词： 牦牛 FGF FGFR 子宫繁殖周期　出处：《中国兽医科学》2017年04期 　论文类型：期刊论文

【摘要】：为探索成纤维细胞因子2(fibroblast growth factor,F2GF2)及成纤维细胞因子受体(fibroblast growth factor receptor,FGFR)在健康成年牦牛繁殖周期中的生物学功能,本研究选取了不同繁殖周期(发情期-卵泡期、发情期-黄体期、妊娠期-黄体期)牦牛子宫组织,采用实时荧光定量PCR(quantitative real-time PCR,RT-qPCR)和免疫组织化学方法从基因和蛋白水平分别检测不同繁殖周期牦牛子宫FGF2和FGFR的表达和分布,用积分光密度方法分析FGF2和FGFR蛋白表达水平。结果显示,不同繁殖周期牦牛子宫中FGF2mRNA表达水平为:发情期-卵泡期发情期-黄体期妊娠期-黄体期(P0.01);FGFRmRNA表达水平为:发情期-黄体期妊娠期-黄体期发情期-卵泡期(P0.05)。FGF2和FGFR在牦牛不同繁殖周期的子宫内膜、子宫腺及血管上皮中均有表达,子宫内膜和子宫腺为主要表达部位,阳性细胞的细胞质中表达较高。积分光密度分析结果显示,FGF2蛋白表达水平为:发情期-卵泡期子宫发情期-黄体期子宫妊娠期-黄体期子宫;FGFR蛋白表达水平为:妊娠期-黄体期子宫发情期-卵泡期子宫发情期-黄体期子宫。以上结果表明,FGF2和FGFR在不同繁殖周期的表达差异性可能与其参与早期胚胎附植及胚胎发育的调控相关,这为进一步研究FGF2和FGFR在牦牛繁殖周期中的调控作用提供了理论依据。
[Abstract]:To explore fibroblast growth factor. F2GF2) and fibroblast growth factor receptor. The biological function of FGFR in the reproductive cycle of healthy adult yaks. In this study, different reproductive cycles (estrus-follicle, estrus-luteal phase, gestational-luteal phase) were selected. Real-time fluorescence quantitative PCR(quantitative real-time PCR was used. RT-qPCR) and immunohistochemical method were used to detect the expression and distribution of FGF2 and FGFR in yak uterus from gene and protein levels, respectively. The expression levels of FGF2 and FGFR proteins were analyzed by integrated optical density method. The expression levels of FGF2mRNA in the uterus of yak in different reproductive cycles were as follows: from estrus to follicle to luteal phase pregnancy to luteal phase P0.01; The expression levels of FGFRmRNA in the endometrium of yaks in different reproductive cycles were as follows: from estrus to luteal phase pregnancy to luteal period to follicular stage. The positive cells were expressed in the cytoplasm of the positive cells. The results of integrated optical density analysis showed that the positive cells were expressed in both uterine gland and vascular epithelium, and the endometrial and uterine glands were the main expression sites. The expression levels of FGF2 protein were: estrus, follicle, estrus, luteal, pregnancy, corpus luteum; The expression level of FGFR protein was: pregnancy luteal phase uterine estrus follicle phase uterine estrus phase luteal phase uterus. The difference of FGF2 and FGFR expression in different reproductive cycles may be related to their involvement in the regulation of early embryo implantation and embryo development. This provides a theoretical basis for further study on the regulation of FGF2 and FGFR in yak reproductive cycle.
【作者单位】：甘肃农业大学动物医学院甘肃省牛羊胚胎工程技术研究中心;
【基金】：国家自然科学基金项目(31272616)
【分类号】：S823.85
【正文快照】： FGF家族具有23种结构多态性生长因子,其中FGF2近些年备受关注。FGF2(fibroblast growthfactor 2)是成纤维细胞生长因子2,属于碱性成纤维细胞生长因子,具有促进心肌细胞增殖和生长,诱导细胞分化,且诱导细胞发生凋亡[1]。在胚胎附植过程中,FGF2在子宫中定位、识别、黏附、附植,

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