不同日龄家兔肠道微生物群落结构

发布时间：2018-01-25 17:16

本文关键词： 断奶幼兔微生物群落热图　出处：《中国兽医学报》2017年09期 　论文类型：期刊论文

【摘要】：采用Miseq2×300测序平台对29,45,60日龄3个阶段的断奶幼兔,共30个粪便样品中的菌群16S rDNA V3和V4可变区进行了高通量测序和分析。结果显示:在不同的日龄间ACE指数和Chao1指数差异显著(P0.05),Shannon指数和Simpson指数差异不显著(P0.05),说明随着日龄的增大断奶兔肠道中的微生物群落丰度大量增加,但是菌群多样性并没有显著增加,表明在幼兔断奶时肠道微生物已经完成了定植。在门水平上,肠道微生物群落主要由厚壁菌门、变形菌门、拟杆菌门、疣微菌门和放线菌门组成,其中变形菌门、拟杆菌门和疣微菌门在幼兔的生长过程中丰度有显著性变化。聚类热图的结果显示断奶幼兔肠道微生物群落结构的变化是随着日龄的变化而逐渐变化。
[Abstract]:Miseq2 脳 300 sequencing platform was used to study the weaning young rabbits at the age of 29 and 45 and 60 days. The 16s rDNA V3 and V4 variable regions in 30 fecal samples were sequenced and analyzed. There were significant differences in ACE index and Chao1 index between different ages (. P0.05). The difference between Shannon index and Simpson index was not significant (P 0.05), which indicated that the abundance of microorganism community in the intestine of weaning rabbits increased significantly with the increase of day age. However, the diversity of microflora did not increase significantly, indicating that intestinal microbes had been colonized during weaning of young rabbits. At gate level, intestinal microbial communities were mainly composed of phylum platyphylum, Proteus and Bacteroides. Microphylum of verruca and actinomycetes are composed of Proteus. The abundance of Bacteroides and Microphylum verruca changed significantly during the growth of young rabbits, and the results of cluster thermogram showed that the changes of intestinal microbial community structure of weaned young rabbits changed gradually with the age of day.
【作者单位】：重庆市畜牧科学院;西南大学动物科学学院;
【基金】：重庆市自然科学基金资助项目(cstc2012jjA80020) 重庆市农发资金资助项目(13407;15405)
【分类号】：S829.1
【正文快照】： 重庆402460)*Corresponding author,E-mail:wangxywang123@163.com家兔是一种依靠肠道内微生物进行消化的单胃草食动物。在家兔的肠道中寄生着种类繁多、数量巨大的微生物,这些数量巨大的微生物个体共同组成了一个微生物群落[1],它可以促进营养的消化吸收,诱导黏膜免疫保护,甚

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