LPS对牛子宫内膜细胞IL-6和IL-8启动子区甲基化状态的影响

发布时间：2018-01-28 02:39

本文关键词： 细菌脂多糖牛子宫内膜细胞 IL- IL- 甲基化　出处：《吉林农业大学学报》2017年05期 　论文类型：期刊论文

【摘要】：为了解细菌脂多糖(Lipopolysaccharide,LPS)诱导牛子宫内膜细胞白介素6(Interleukin 6,IL-6)和白介素8(Interleukin 8,IL-8)mRNA表达上调的分子机制,利用亚硫酸氢测序(Bisulfite sequencing PCR,BSP)技术分析LPS对牛子宫内膜细胞IL-6和IL-8基因启动子区甲基化状态的影响,明确DNA甲基化对LPS诱导的牛子宫内膜细胞IL-6和IL-8基因表达的调控作用。用1μg/m L的LPS处理牛子宫内膜细胞24 h,用BSP技术检测IL-6和IL-8表达量及其启动子区甲基化水平。结果表明:LPS可显著提高IL-6和IL-8表达量,伴随着IL-6和IL-8启动子区DNA甲基化水平降低,尤其在IL-6启动子区-660及IL-8启动子区-120和-48位点降低最明显。结果提示,LPS可降低牛子宫内膜细胞IL-6和IL-8启动子特定位点甲基化水平。
[Abstract]:In order to understand the bacterial lipopolysaccharide (Lipopolysaccharide, LPS) induced by bovine endometrial cells interleukin 6 (Interleukin 6, IL-6) and interleukin 8 (Interleukin 8, IL-8) mRNA expression of molecular mechanisms regulating the use of bisulfite sequencing (Bisulfite sequencing, PCR, BSP) technical analysis of effect of LPS on bovine endometrial cells IL-6 and IL-8 gene promoter methylation, clear DNA methylation regulation effect on the expression of IL-6 and IL-8 in bovine endometrial cells induced by LPS gene. Using 1 g/m L LPS 24 h bovine endometrial cells, expression levels and its promoter region was detected by BSP IL-6 and IL-8. The results showed that LPS significantly increased IL-6 and IL-8 expression, accompanied by IL-6 and IL-8 promoter DNA methylation decreased the levels of the sub area, especially in the promoter region of IL-6 -660 and IL-8 -120 in the promoter region and -48 sites decreased most significantly. The results suggest that LPS can reduce cattle The methylation level of IL-6 and IL-8 promoters in endometrium cells.

【作者单位】：吉林农业大学动物科学技术学院;吉林省科技创新平台管理中心;
【基金】：国家自然科学基金面上项目(31672420) 吉林省科技厅项目(20150519018JH,20150204074NY,20160209001NY)
【分类号】：S823
【正文快照】： 130012引文格式:王军,燕晓晓,张涛,等.LPS对牛子宫内膜细胞IL-6和IL-8启动子区甲基化状态的影响[J].吉林农业大学学报,2017,39(5):603-607.***crease methylation levels at specific Cp G sites in IL-6 and IL-8 promoter regions.子宫内膜炎是造成奶牛繁殖障碍的主要原因,

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