鲜菌草与灵芝菌丝发酵菌质多糖提取工艺及其体外抗氧化性

发布时间：2018-01-28 18:25

  本文关键词： 菌草 灵芝菌丝 多糖 提取工艺 清除率　出处：《北方园艺》2017年09期 　论文类型：期刊论文

【摘要】：以鲜菌草与灵芝菌丝发酵得到的菌质为试材,采用单因素试验和正交实验对其多糖进行提取,研究了不同的料液比、提取时间、提取温度和提取次数对菌质多糖提取率的影响,获得最优化的提取条件;用Fenton体系和邻苯三酚自氧化体系研究了菌质多糖对羟自由基和超氧自由基清除率的影响。结果表明:影响鲜菌草与灵芝菌丝发酵菌质多糖提取得率的主次因素分别为提取温度料液比提取次数提取时间;最佳提取工艺条件为料液比1∶30g·mL~(-1)、温度100℃、提取时间4h、提取次数3次,以此最佳组合提取鲜菌草与灵芝菌丝发酵菌质多糖时,其得率为4.88%。鲜菌草与灵芝菌丝发酵菌质多糖对羟基自由基和超氧阴离子自由基有明显的清除作用,并且随着多糖浓度的增加,清除能力增强,表明鲜菌草与灵芝菌丝发酵菌质多糖对羟基自由基和超氧阴离子自由基的清除能力与多糖浓度有明显的量效关系。通过相关方程可以得到鲜菌草与灵芝菌丝发酵菌质多糖清除羟基自由基的EC_(50)值为0.461mg·mL~(-1),清除超氧阴离子自由基的EC_(50)值为0.864mg·mL~(-1)。
[Abstract]:The polysaccharides from fresh fungus and Ganoderma lucidum were extracted by single factor experiment and orthogonal experiment. The different ratio of material to liquid and extraction time were studied. The effects of extraction temperature and extraction times on the extraction rate of polysaccharides were studied and the optimum extraction conditions were obtained. The effects of mycelia polysaccharides on the scavenging rates of hydroxyl radical and superoxide free radical were studied by Fenton system and pyrogallol autooxidation system. The main and secondary factors affecting the extraction rate of polysaccharides from fresh fungus and Ganoderma lucidum fermentation were the extraction time of extracting temperature ratio of material to liquid. The optimum extraction conditions were as follows: ratio of material to liquid 1: 30 g 路mL ~ (-1), temperature 100 鈩，

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