捕食线虫性真菌-少孢节丛孢菌冻干生物制剂的制备

发布时间：2018-01-30 00:12

本文关键词： 捕食线虫性真菌少孢节丛孢菌孢子培养冻干制剂　出处：《中国兽医学报》2017年08期 　论文类型：期刊论文

【摘要】：通过在含有0.05%琼脂粉的沙氏葡萄糖肉汤培养基中培养少孢节丛孢菌(Arthrobotrys oligospora)再转接到玉米粒培养基的方法,对少孢节丛孢菌分生孢子进行培养。在不同时间点,将培养好的分生孢子从培养基上洗脱并进行计数,然后制成冻干制剂,并观察其复苏情况,从而为少孢节丛孢菌冻干制剂的临床应用提供基础数据。结果显示:通过对少孢节丛孢菌冻干制剂在萌发率、生长率和捕食率三方面的复苏情况观察,少孢节丛孢菌冻干后的分生孢子在接种后4d,萌发率达到最大值79.5%,平均生长率为3.4mm/d,加入虫体后7d捕食率最高,为95.8%,平均每天捕食率为74.0%;少孢节丛孢菌冻干制剂在平均生长率和捕食率方面与对照组相比,差异均不显著(P0.1)。结果表明,冻干制剂材料易得,方法简单易行,复苏效果良好,进一步优化后将具有产业化应用的前景。
[Abstract]:Arthrobotrys oligospora was cultured in a broth medium containing 0.05% Agar powder. The method of transferring to the corn kernel medium. At different time points, the cultured conidia were eluted from the culture medium and counted, then the lyophilized preparation was prepared and its recovery was observed. The results showed that the recovery rate, growth rate and predation rate of lyophilized preparation were observed in three aspects: germination rate, growth rate and predation rate. The germination rate of the lyophilized conidia reached a maximum of 79.5 mm / d at 4 days after inoculation, and the average growth rate was 3.4 mm / d, and the predation rate was the highest (95.8%) at 7 days after inoculation. The average daily predation rate was 74.0%; Compared with the control group, the average growth rate and predation rate of the lyophilized preparation were not significantly different from those of the control group (P 0.1). The results showed that the freeze-dried preparation was easy to obtain, the method was simple and feasible, and the effect of resuscitation was good. Further optimization will have the prospect of industrial application.
【作者单位】：内蒙古农业大学兽医学院农业部动物疾病临床诊疗技术重点实验室;
【基金】：公益性行业(农业)科研专项基金资助项目(201303037) 内蒙古自然科学基金资助项目(2015MS0308) 家畜疫病病原生物学国家重点实验室开放基金资助项目(SKLVEB2015KFKT013)
【分类号】：S859.5
【正文快照】： 家畜寄生性线虫病在家畜中普遍存在,并且具2006年,内蒙古农业大学兽医寄生虫学实验室在国有分布广、感染率高、危害大的特点[1]。由于各种线内首先开始研究了少孢节丛孢菌(A.oligospora)的虫广泛寄生于家畜体内,吸收营养物质、损害健康冻干制剂,并且将其冷冻放置3年后,又将其

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