SPF雏鸡感染REV后中枢免疫器官细胞凋亡及相关因子的变化

发布时间：2018-01-31 08:11

本文关键词： 禽网状内皮组织增生病病毒 Caspase- Caspase- Bcl- 细胞凋亡　出处：《畜牧兽医学报》2017年10期 　论文类型：期刊论文

【摘要】：为探索禽网状内皮组织增生病病毒(REV)感染对雏鸡中枢免疫器官细胞凋亡及其相关酶活性和基因表达的影响,将70只1日龄SPF雏鸡随机分为REV感染组(I)和对照组(C),应用酶活性检测、荧光定量PCR、ELISA、TUNEL等方法对中枢免疫器官细胞的Caspase-3和Caspase-9活性、Bcl-2mRNA及其蛋白质表达和凋亡细胞的动态变化进行了检测。结果发现,SPF雏鸡感染REV后,其胸腺中Caspase-3和Caspase-9活性、Bcl-2mRNA及其蛋白质含量、凋亡细胞数量均于7~28d内不同程度(P0.05或P0.01)高于对照组雏鸡;法氏囊中上述被检指标均于7~35d内显著(P0.05)或极显著(P0.01)高于对照组雏鸡。研究表明REV感染引起SPF雏鸡中枢免疫器官Caspase-3和Caspase-9活性增强、Bcl-2mRNA及其蛋白质表达上调所致的细胞凋亡与雏鸡免疫器官功能抑制密切相关。
[Abstract]:To investigate the effects of avian reticuloendotheliosis virus (Reva) infection on the apoptosis of central immune organs and related enzyme activity and gene expression in chicks. Seventy 1-day-old SPF chicks were randomly divided into REV infected group and control group. The activities of Caspase-3 and Caspase-9 in central immune organ cells were studied by TUNEL and other methods. The expression of Bcl-2mRNA and its protein and the dynamic changes of apoptotic cells were detected. The Caspase-3 and Caspase-9 activity of Bcl-2 mRNA and its protein content in the thymus were observed. The number of apoptotic cells was higher than that of the control group in different degree (P0.05 or P0.01). In the bursa of Fabricius, the above detected indexes were significant within 75 days (P0.05) or extremely significant (P0.01). The results showed that the activities of Caspase-3 and Caspase-9 in the central immune organs of SPF chicks were increased by REV infection. Apoptosis induced by upregulation of Bcl-2mRNA and its protein is closely related to inhibition of immune organ function in chicks.
【作者单位】：东北农业大学动物医学学院黑龙江省实验动物与比较医学重点实验室;秦皇岛圣蓝皇家海洋公园;
【基金】：国家自然科学基金(31472169)
【分类号】：S858.31
【正文快照】： 禽网状内皮组织增生病(reticuloendotheliosis,RE)是由禽网状内皮组织增生病病毒(REV)引起的综合征,包括急性网状细胞增生症、矮小综合征和淋巴组织以及其他组织的慢性肿瘤增生性疾病。RE是继鸡淋巴细胞性白血病、鸡马立克病之后的第三种禽病毒性肿瘤病[1]。近年来,国内REV的

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