荷斯坦青年种公牛免疫应答能力分析

发布时间：2018-01-31 13:30

本文关键词： 免疫应答能力细胞免疫体液免疫淋巴细胞增殖支原体　出处：《河北工程大学》2017年硕士论文　论文类型：学位论文

【摘要】：免疫系统是机体执行免疫应答及免疫功能的重要系统。有研究发现,免疫反应有25%的遗传力。这一遗传力和生产性能及体型遗传力相类似,充分利用免疫应答能力较高的公牛,可以提高公犊利用率,使牛群整体免疫力普遍提高,并且可将这些更高免疫反应遗传给后代。本论文以优良荷斯坦犊公牛为研究对象,针对细胞免疫和抗体免疫,采用流式细胞术、荧光定量PCR、淋巴细胞增殖及ELISA液相阻断抗体检测等试验方法,分析不同个体免疫应答能力。根据其免疫应答水平分为高免疫应答个体与低免疫应答个体,并对高免疫应答能力个体及低免疫应答能力个体间的差异性进行分析。体液免疫试验通过ELISA液相阻断抗体检测不同个体免疫刺激后血清中抗体含量。结果表明,不同个体间抗体含量差异显著;不同免疫时间其抗体含量差异较显著。母源抗体较高的个体其一免刺激后抗体效价出现明显下降;根据群体抗体效价筛选高抗体介导的免疫应答个体。口蹄疫三种亚型间抗体效价较高的个体与抗体效价较低的个体进行分析比较,A型抗体效价免疫刺激各阶段差异较显著。细胞免疫试验当中,通过T淋巴亚群CD4~+CD8~+检测,发现群体免疫前后表达率差异不显著;经ConA刺激后,不同个体间表达率差异显著;结果表明,增殖率较高的个体其免疫应答能力较高,增殖率较低的个体其免疫应答能力较低;荧光定量PCR试验使用T细胞有丝分裂原ConA和B细胞有丝分裂原LPS分别刺激后测定其Th1型细胞因子IL-2、IFN-γ和Th2型细胞因子IL-4、IL-6的表达量。结果表明,不同个体间免疫因子的表达率存在显著差异;ConA和LPS刺激后差异不显著。综合分析个体间细胞免疫应答能力,筛选免疫应答较高与较低的个体。本研究采用牛支原体作为感染病原,对高免组和低免组的分组依据进行验证。结果表明,支原体能够干扰宿主的免疫应答能力,对于免疫力较高的群体,支原体感染对宿主免疫应答能力的干扰较弱,而对于免疫低的群体较强。
[Abstract]:The immune system is an important system for the body to carry out immune response and immune function. Some studies have found that the heritability of immune response is 25%. This heritability is similar to the heritability of production performance and somatotype. Making full use of the bull with high immune response ability can improve the utilization rate of bull and improve the whole immunity of cattle. And these higher immune responses can be inherited to the offspring. In this paper, the fine Holstein calf as the study object, cellular immunity and antibody immunization, flow cytometry, fluorescence quantitative PCR. Lymphocyte proliferation and ELISA liquid phase blocking antibody assay were used to analyze the immune response ability of different individuals. According to their immune response level, they were divided into high immune response individuals and low immune response individuals. The differences between individuals with high immune response ability and those with low immune response ability were analyzed. Humoral immunoassay was used to detect the serum antibody content of different individuals after immune stimulation by ELISA liquid phase blocking antibody. The fruit shows. There were significant differences in antibody content among different individuals. The antibody titers of one of the individuals with higher maternal antibody were significantly decreased after immune stimulation. High antibody mediated immune response individuals were screened according to the antibody titer. The higher antibody titers among the three subtypes of foot-and-mouth disease were compared with those with lower antibody titers. There were significant differences in the titer of type A antibody in different stages of immunostimulation. In the cellular immunity test, the expression rate of CD4 ~ CD8 ~ in T lymphocyte subsets was not significantly different before and after immunization. After ConA stimulation, there were significant differences in expression rates among different individuals. The results showed that the individuals with higher proliferation rate had higher immune response ability and those with lower proliferation rate had lower immune response ability. Fluorescence quantitative PCR test was used to detect the Th1 type IL-2 of T cell mitogen ConA and B cell mitogen LPS after stimulation. The expression of IFN- 纬 and Th2 cytokine IL-4 and IL-6. The results showed that there were significant differences in the expression rate of immune factors among different individuals. There was no significant difference between ConA and LPS after stimulation. The ability of cellular immune response between individuals was analyzed synthetically to screen the individuals with high and low immune response. Mycoplasma bovis was used as the pathogen of infection in this study. The results showed that mycoplasma could interfere with the immune response ability of the host. Mycoplasma infection interfered weakly with host immune response, but was stronger in low immune population.
【学位授予单位】：河北工程大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S823

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