木犀草素联合庆大霉素对耐药化脓隐秘杆菌抑制作用的研究

发布时间：2018-01-31 17:21

本文关键词： 木犀草素化脓隐秘杆菌药物联合抗菌作用逆转耐药　出处：《中国兽药杂志》2016年11期 　论文类型：期刊论文

【摘要】：为探究木犀草素联合庆大霉素对耐药化脓隐秘杆菌的抑制作用,采用微量肉汤稀释法分别测定木犀草素和庆大霉素对34株化脓隐秘杆菌的最小抑菌浓度(MIC)。选取对庆大霉素耐药的化脓隐秘杆菌,通过棋盘法测定两种药物联合抑菌效果。经亚抑菌浓度木犀草素作用耐药菌株,比较木犀草素作用前后化脓隐秘杆菌对庆大霉素的耐药性变化情况。结果表明,木犀草素对化脓隐秘杆菌的MIC值为39~156μg/m L,与庆大霉素联合的作用为不相关;耐药菌经亚抑菌浓度木犀草素(1/4MIC)作用36 h后,庆大霉素对耐药菌的MIC值下降2~32倍,且原菌株耐药水平越高,MIC下降倍数越高。提示木犀草素具有良好抗菌活性,且能够逆转化脓隐秘杆菌耐药性。
[Abstract]:To investigate the inhibitory effect of luteolin combined with gentamicin on drug-resistant covert bacillus pyogenes. The minimal inhibitory concentration of luteolin and gentamicin against 34 strains of mycobacterium pyogenes was determined by broth dilution method. The antimicrobial effect of two drugs combined with luteolin was determined by chessboard method. The resistance of covert bacilli to gentamicin was compared before and after the treatment of luteolin by subinhibitory concentration of luteolin. The results showed that the drug resistance of mycobacterium pyogenes to gentamicin was higher than that of luteolin. The MIC value of luteolin was 390.156 渭 g / mL, and the effect of luteolin was not related to gentamicin. The MIC value of gentamicin to resistant bacteria decreased by 2 ~ 32 times after treatment with luteolin (1 / 4 MICM) for 36 h, and the drug resistance level of the original strain was higher than that of the original strain. It suggested that luteolin had good antimicrobial activity and could reverse the drug resistance of Stereobacterium pyogenes.
【作者单位】：沈阳农业大学畜牧兽医学院;建平县畜产品安全监督所;
【分类号】：S852.61
【正文快照】： 近年来,细菌的耐药问题日趋严重,耐药水平越来越高,给养殖业发展造成极大困扰[1]。新型抗菌药物和逆转细菌耐药性药物制剂的研发已成为学者研究的热点。我国传统中药不但具有广谱抗菌作用,还能够逆转细菌耐药,可以减轻细菌的选择压力,从而不易产生耐药性[2]。木犀草素作为天然

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