2014年-2016年广西地区猪繁殖与呼吸综合征病毒Nsp2和ORF5基因的遗传多样性分析

发布时间：2018-01-31 22:11

  本文关键词： 猪繁殖与呼吸综合征病毒 Nsp基因 ORF基因 遗传多样性　出处：《动物医学进展》2017年01期 　论文类型：期刊论文

【摘要】：为了解广西地区猪繁殖与呼吸综合征病毒(PRRSV)流行毒株的遗传进化情况,对2014年-2016年来自广西各地的部分PRRSV阳性病料进行Nsp2和ORF5基因的扩增和测序分析。结果获得34个Nsp2基因序列和45个ORF5基因序列,均属于美洲型毒株。Nsp2基因间核苷酸序列的同源性为91.8%~100%,与PRRSV美洲型毒株VR-2332、CH-1a、JXA1及NADC30株核苷酸序列的同源性分别为81.3%~84.3%、88.9%~92.1%、94.3%~99.3%和73.5%~75.1%,而与PRRSV欧洲型毒株LV株核苷酸序列的同源性为51.5%~53.2%。ORF5基因间核苷酸序列的同源性为82.8%~100%,与PRRSV美洲型毒株VR-2332、CH-1a、JXA1及NADC30株核苷酸序列的同源性分别为83.7%~99.5%、85%~95%、83.8%~99.7%和83.2%~86.4%,而与PRRSV欧洲型毒株LV株核苷酸序列的同源性为62.4%~64.5%。基于Nsp2和ORF5基因推导的氨基酸序列绘制的遗传进化树中,广西地区的毒株主要分布在以JXA1为代表的Ⅳ亚群。表明当前广西PRRSV流行毒株以JXA1株为代表的高致病性美洲型毒株为主,各毒株Nsp2和ORF5基因序列存在一定的差异,尚未发现欧洲型毒株和美洲型NADC30类毒株。
[Abstract]:To understand the genetic evolution of porcine reproductive and respiratory syndrome virus (PRRSVV) epidemic strains in Guangxi. From 2014 to 2016, some samples of PRRSV positive venereal diseases from various parts of Guangxi were amplified and sequenced by Nsp2 and ORF5. Results 34 Nsp2 gene sequences and 45% Nsp2 gene sequences were obtained. A sequence of ORF5 genes. The homology of nucleotide sequence between the two strains was 91.8100, which was similar to that of PRRSV strain VR-2332 (CH-1a). The nucleotide sequence of JXA1 and NADC30 strains were 81.3% and 84.3%, respectively. 94.3% and 73.5% respectively. The nucleotide sequence homology with LV strain of PRRSV was 51.5%. The homology of ORF5 gene sequence was 82.8%. The homology of nucleotide sequence with PRRSV strain VR-2332CH-1aA1and NADC30 strain was 83.7% and 99.585%, respectively. 83.8% and 83.2%, respectively. The nucleotide sequence homology with PRRSV European strain LV strain was 62.4% and 64.5%. The amino acid sequences derived from Nsp2 and ORF5 genes were mapped in the phylogenetic tree. The distribution of the virus strains in Guangxi was mainly in the 鈪，

本文编号：1480117